N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Cristina De Castro; Immacolata Speciale; Garry Duncan; David D. Dunigan; Irina Agarkova; Rosa Lanzetta; Luisa Sturiale; Angelo Palmigiano; Domenico Garozzo; Antonio Molinaro; Michela Tonetti; James L. Van Etten

doi:10.1002/anie.201509150

N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Cristina De Castro, Immacolata Speciale, Garry Duncan, David D. Dunigan, Irina Agarkova, Rosa Lanzetta, Luisa Sturiale, Angelo Palmigiano, Domenico Garozzo, Antonio Molinaro, Michela Tonetti, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

Original language	English (US)
Pages (from-to)	654-658
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	2
DOIs	https://doi.org/10.1002/anie.201509150
State	Published - Jan 11 2016

Keywords

glycoproteins
glycosylations
natural products
structural biology
viruses

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201509150

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title = "N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent",

abstract = "N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.",

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author = "{De Castro}, Cristina and Immacolata Speciale and Garry Duncan and Dunigan, {David D.} and Irina Agarkova and Rosa Lanzetta and Luisa Sturiale and Angelo Palmigiano and Domenico Garozzo and Antonio Molinaro and Michela Tonetti and {Van Etten}, {James L.}",

note = "Funding Information: Work in the Van Etten laboratory was partially supported by National Science Foundation grant EPS-1004094, Stanley Medical Research Institute grant 11R-003, and the National Institute of Health grant P20-RR15635 from the Centers of Biomedical Research Excellence Program of the National Center for Research Resources. G.D. was supported by National Institute for General Medical Science grant P20GM103427. Work in the Garozzo laboratory was partially supported by Stellalucente Trust and the Italian National Council of Research (CNR). C.D.C. and A.M. wish to acknowledge financial support from NMR IR-RMN-THC Fr3050 CNRS for the use of 950 MHz NMR spectrometer. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

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doi = "10.1002/anie.201509150",

language = "English (US)",

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journal = "Angewandte Chemie - International Edition",

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T1 - N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

AU - De Castro, Cristina

AU - Speciale, Immacolata

AU - Duncan, Garry

AU - Dunigan, David D.

AU - Agarkova, Irina

AU - Lanzetta, Rosa

AU - Sturiale, Luisa

AU - Palmigiano, Angelo

AU - Garozzo, Domenico

AU - Molinaro, Antonio

AU - Tonetti, Michela

AU - Van Etten, James L.

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PY - 2016/1/11

Y1 - 2016/1/11

N2 - N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

AB - N-glycosylation is a fundamental modification of proteins and exists in the three domains of life and in some viruses, including the chloroviruses, for which a new type of core N-glycan is herein described. This N-glycan core structure, common to all chloroviruses, is a pentasaccharide with a β-glucose linked to an asparagine residue which is not located in the typical sequon N-X-T/S. The glucose is linked to a terminal xylose unit and a hyperbranched fucose, which is in turn substituted with a terminal galactose and a second xylose residue. The third position of the fucose unit is always linked to a rhamnose, which is a semiconserved element because its absolute configuration is virus-dependent. Additional decorations occur on this core N-glycan and represent a molecular signature for each chlorovirus.

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KW - glycosylations

KW - natural products

KW - structural biology

KW - viruses

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N-Linked Glycans of Chloroviruses Sharing a Core Architecture without Precedent

Abstract

Keywords

ASJC Scopus subject areas

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