Nanocarrier stimuli-activated gene delivery

The nanoscale formation and activation of stimuli-responsive systems poly(N-isoproplyacrylamide)(PNIPA)- polyethylene(PEI)/DNA systems, was investigated using atomic force microscopy technique. The extracellular and intracellular behavior of nanocarriers was analyzed in response to thermal and redox stimuli. The thermoresponsive copolymers were prepared using PNIPA and single terminal carboxylic acid group conjugated with PEI. The relative content of NLS and HRP peptides in the synthesized copolypeptides was determined by amino acid analysis. Plasmid DNA/PEI-NIPA polyplexes nanocarriers were prepared with sodium acetate buffer (pH 5) by adding peptide solution into 150 mL of DNA in sodium acetate buffer. Nanocarriers were imagined using AFM under atmospheric conditions. It was observed that thermoresponsive nanocarriers showed increase in their size for site-specific deposition. Result shows that nanocarriers dissemble into the constituent components in intracellular condition.