Nanocarrier stimuli-activated gene delivery

Kenneth A. Howard, Mingdong Dong, David Oupicky, Harender S. Bisht, Claus Buss, Flemming Besenbacher, Jørgen Kjems

Research output: Contribution to journalReview article

39 Scopus citations

Abstract

The nanoscale formation and activation of stimuli-responsive systems poly(N-isoproplyacrylamide)(PNIPA)- polyethylene(PEI)/DNA systems, was investigated using atomic force microscopy technique. The extracellular and intracellular behavior of nanocarriers was analyzed in response to thermal and redox stimuli. The thermoresponsive copolymers were prepared using PNIPA and single terminal carboxylic acid group conjugated with PEI. The relative content of NLS and HRP peptides in the synthesized copolypeptides was determined by amino acid analysis. Plasmid DNA/PEI-NIPA polyplexes nanocarriers were prepared with sodium acetate buffer (pH 5) by adding peptide solution into 150 mL of DNA in sodium acetate buffer. Nanocarriers were imagined using AFM under atmospheric conditions. It was observed that thermoresponsive nanocarriers showed increase in their size for site-specific deposition. Result shows that nanocarriers dissemble into the constituent components in intracellular condition.

Original languageEnglish (US)
Pages (from-to)54-57
Number of pages4
JournalSmall
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2007

    Fingerprint

Keywords

  • Atomic force microscopy
  • DNA
  • Gene delivery
  • Nanocarriers
  • Polymers

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Howard, K. A., Dong, M., Oupicky, D., Bisht, H. S., Buss, C., Besenbacher, F., & Kjems, J. (2007). Nanocarrier stimuli-activated gene delivery. Small, 3(1), 54-57. https://doi.org/10.1002/smll.200600328