TY - JOUR
T1 - Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering
AU - Quint, Jacob P.
AU - Samandari, Mohamadmahdi
AU - Abbasi, Laleh
AU - Mollocana, Evelyn
AU - Rinoldi, Chiara
AU - Mostafavi, Azadeh
AU - Tamayol, Ali
N1 - Funding Information:
The financial support from the National Institutes of Health (AR077132, AR073822, AR079114) and the University of Connecticut are gratefully acknowledged. C. R. would like to acknowledge the financial support from the Polish Ministry of Science and Higher Education through the scholarship for outstanding young scientists and from the Foundation for Polish Science (FNP).
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2022/1/21
Y1 - 2022/1/21
N2 - Extreme loss of skeletal muscle overwhelms the natural regenerative capability of the body, results in permanent disability and substantial economic burden. Current surgical techniques result in poor healing, secondary injury to the autograft donor site, and incomplete recuperation of muscle function. Most current tissue engineering and regenerative strategies fail to create an adequate mechanical and biological environment that enables cell infiltration, proliferation, and myogenic differentiation. In this study, we present a nanoengineered skeletal muscle scaffold based on functionalized gelatin methacrylate (GelMA) hydrogel, optimized for muscle progenitors' proliferation and differentiation. The scaffold was capable of controlling the release of insulin-like growth factor 1 (IGF-1), an important myogenic growth factor, by utilizing the electrostatic interactions with LAPONITE® nanoclays (NCs). Physiologically relevant levels of IGF-1 were maintained during a controlled release over two weeks. The NC was able to retain 50% of the released IGF-1 within the hydrogel niche, significantly improving cellular proliferation and differentiation compared to control hydrogels. IGF-1 supplemented medium controls required 44% more IGF-1 than the comparable NC hydrogel composites. The nanofunctionalized scaffold is a viable option for the treatment of extreme muscle injuries and offers scalable benefits for translational interventions and the growing field of clean meat production.
AB - Extreme loss of skeletal muscle overwhelms the natural regenerative capability of the body, results in permanent disability and substantial economic burden. Current surgical techniques result in poor healing, secondary injury to the autograft donor site, and incomplete recuperation of muscle function. Most current tissue engineering and regenerative strategies fail to create an adequate mechanical and biological environment that enables cell infiltration, proliferation, and myogenic differentiation. In this study, we present a nanoengineered skeletal muscle scaffold based on functionalized gelatin methacrylate (GelMA) hydrogel, optimized for muscle progenitors' proliferation and differentiation. The scaffold was capable of controlling the release of insulin-like growth factor 1 (IGF-1), an important myogenic growth factor, by utilizing the electrostatic interactions with LAPONITE® nanoclays (NCs). Physiologically relevant levels of IGF-1 were maintained during a controlled release over two weeks. The NC was able to retain 50% of the released IGF-1 within the hydrogel niche, significantly improving cellular proliferation and differentiation compared to control hydrogels. IGF-1 supplemented medium controls required 44% more IGF-1 than the comparable NC hydrogel composites. The nanofunctionalized scaffold is a viable option for the treatment of extreme muscle injuries and offers scalable benefits for translational interventions and the growing field of clean meat production.
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U2 - 10.1039/d1nr06143g
DO - 10.1039/d1nr06143g
M3 - Article
C2 - 34951427
AN - SCOPUS:85123642961
SN - 2040-3364
VL - 14
SP - 797
EP - 814
JO - Nanoscale
JF - Nanoscale
IS - 3
ER -