Nanofiber-expanded human umbilical cord blood-derived CD34+ cell therapy accelerates murine cutaneous wound closure by attenuating pro-inflammatory factors and secreting IL-10

Suman Kanji, Manjusri Das, Reeva Aggarwal, Jingwei Lu, Matthew Joseph, Sujit Basu, Vincent J. Pompili, Hiranmoy Das

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Nanofiber-expanded human umbilical cord blood-derived CD34. + cell therapy is under consideration for treating peripheral and cardiac ischemia. However, the therapeutic efficacy of nanofiber-expanded human umbilical cord blood-derived (NEHUCB) CD34. + cell therapy for wound healing and its mechanisms are yet to be established. Using an excision wound model in NOD/SCID mice, we show herein that NEHUCB-CD34. + cells home to the wound site and significantly accelerate the wound-healing process compared to vehicle-treated control. Histological analysis reveals that accelerated wound closure is associated with the re-epithelialization and increased angiogenesis. Additionally, NEHUCB-CD34. + cell-therapy decreases expression of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-6 and NOS2A in the wound bed, and concomitantly increases expression of IL-10 compared to vehicle-treated control. These findings were recapitulated in vitro using primary dermal fibroblasts and NEHUCB-CD34. + cells. Moreover, NEHUCB-CD34. + cells attenuate NF-κB activation and nuclear translocation in dermal fibroblasts through enhanced secretion of IL-10, which is known to bind to NF-κB and suppress transcriptional activity. Collectively, these data provide novel mechanistic evidence of NEHUCB-CD34. + cell-mediated accelerated wound healing.

Original languageEnglish (US)
Pages (from-to)275-288
Number of pages14
JournalStem Cell Research
Volume12
Issue number1
DOIs
StatePublished - Jan 2014

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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