Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain

Xiaowei Li, Stephany Y. Tzeng, Xiaoyan Liu, Markus Tammia, Yu Hao Cheng, Andrew Rolfe, Dong Sun, Ning Zhang, Jordan J. Green, Xuejun Wen, Hai Quan Mao

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.

Original languageEnglish (US)
Pages (from-to)157-166
Number of pages10
JournalBiomaterials
Volume84
DOIs
StatePublished - Apr 1 2016

Keywords

  • Nanoparticles
  • Neurogenin-2
  • Neuronal differentiation
  • Transfection
  • Traumatic brain injury

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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