Nanoscale Dynamics of Amyloid β-42 Oligomers As Revealed by High-Speed Atomic Force Microscopy

Siddhartha Banerjee, Zhiqiang Sun, Eric Y. Hayden, David B. Teplow, Yuri L. Lyubchenko

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Amyloid β-protein (Aβ) oligomers are emerging as potent neurotoxic species in Alzheimer's disease pathogenesis. Detailed characterization of oligomer structure and dynamics is necessary to develop oligomer-specific therapeutic agents. However, oligomers exist transiently, which complicates their structural analysis. One approach to mitigate these problems has been photochemical cross-linking of native oligomers. In these states, the oligomers can be isolated and purified for physical and chemical studies. Here we characterized the structure of isolated cross-linked Aβ42 trimers, pentamers, and heptamers with atomic force microscopy (AFM) imaging and probed their dynamics in solution using time-lapse high-speed AFM. This technique enables visualization of the structural dynamics of the oligomers at nanometer resolution on a millisecond time scale. Results demonstrate that cross-linked pentamers and heptamers are very dynamic fluctuating between a compact single-globular and multiglobular assemblies. Trimers remain in their single-globular geometry that elongates adopting an ellipsoidal shape. Biological significance of oligomers dynamics is discussed.

Original languageEnglish (US)
Pages (from-to)12202-12209
Number of pages8
JournalACS Nano
Volume11
Issue number12
DOIs
StatePublished - Dec 26 2017

Keywords

  • AFM time lapse
  • Alzheimer's disease
  • amyloid oligomers
  • amyloid β-protein
  • atomic force microscopy
  • single-molecule dynamics

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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