Nanoscale nucleosome dynamics assessed with time-lapse AFM

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations

Abstract

A fundamental challenge associated with chromosomal gene regulation is accessibility of DNA within nucleosomes. Recent studies performed by various techniques, including single-molecule approaches, led to the realization that nucleosomes are dynamic structures rather than static systems, as was once believed. Direct data are required in order to understand the dynamics of nucleosomes more clearly and to answer fundamental questions, including: What is the range of nucleosome dynamics? Does a non-ATP-dependent unwrapping process of nucleosomes exist? What are the factors facilitating the large-scale opening and unwrapping of nucleosomes? This review summarizes the results of nucleosome dynamics obtained with time-lapse AFM, including a high-speed version (HS-AFM) capable of visualizing molecular dynamics on the millisecond time scale. With HS-AFM, the dynamics of nucleosomes at a sub-second time scale was observed, allowing one to visualize various pathways of nucleosome dynamics, such as sliding and unwrapping, including complete dissociation. Overall, these findings reveal new insights into the dynamics of nucleosomes and the novel mechanisms controlling spontaneous chromatin dynamics.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalBiophysical Reviews
Volume6
Issue number2
DOIs
StatePublished - Jun 2014

Keywords

  • Atomic force microscopy
  • Chromatin dynamics
  • Nanoimaging
  • Nucleosomes dynamics
  • Single-molecule biophysics

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology

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