TY - JOUR
T1 - Nanosecond pulsed electric field induced changes in cell surface charge density
AU - Dutta, Diganta
AU - Palmer, Xavier Lewis
AU - Asmar, Anthony
AU - Stacey, Michael
AU - Qian, Shizhi
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/9/1
Y1 - 2017/9/1
N2 - This study reports that the surface charge density changes in Jurkat cells with the application of single 60 nanosecond pulse electric fields, using atomic force microscopy. Using an atomic force microscope tip and Jurkat cells on silica in a 0.01 M KCl ionic concentration, we were able to measure the interfacial forces, while also predicting surface charge densities of both Jurkat cell and silica surfaces. The most important finding is that the pulsing conditions varyingly reduced the cells’ surface charge density. This offers a novel way in which to examine cellular effects of pulsed electric fields that may lead to the identification of unique mechanical responses. Compared to a single low field strength NsPEF (15 kV/cm) application, exposure of Jurkat cells to a single high field strength NsPEF (60 kV/cm) resulted in a further reduction in charge density and major morphological changes. The structural, physical, and chemical properties of biological cells immensely influence their electrostatic force; we were able to investigate this through the use of atomic force microscopy by measuring the surface forces between the AFM's tip and the Jurkat cells under different pulsing conditions as well as the interfacial forces in ionic concentrations.
AB - This study reports that the surface charge density changes in Jurkat cells with the application of single 60 nanosecond pulse electric fields, using atomic force microscopy. Using an atomic force microscope tip and Jurkat cells on silica in a 0.01 M KCl ionic concentration, we were able to measure the interfacial forces, while also predicting surface charge densities of both Jurkat cell and silica surfaces. The most important finding is that the pulsing conditions varyingly reduced the cells’ surface charge density. This offers a novel way in which to examine cellular effects of pulsed electric fields that may lead to the identification of unique mechanical responses. Compared to a single low field strength NsPEF (15 kV/cm) application, exposure of Jurkat cells to a single high field strength NsPEF (60 kV/cm) resulted in a further reduction in charge density and major morphological changes. The structural, physical, and chemical properties of biological cells immensely influence their electrostatic force; we were able to investigate this through the use of atomic force microscopy by measuring the surface forces between the AFM's tip and the Jurkat cells under different pulsing conditions as well as the interfacial forces in ionic concentrations.
KW - Atomic force microscopy
KW - Jurkat cell
KW - Surface charge density
UR - http://www.scopus.com/inward/record.url?scp=85018384110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018384110&partnerID=8YFLogxK
U2 - 10.1016/j.micron.2017.04.009
DO - 10.1016/j.micron.2017.04.009
M3 - Article
C2 - 28494437
AN - SCOPUS:85018384110
SN - 0968-4328
VL - 100
SP - 45
EP - 49
JO - Micron
JF - Micron
ER -