Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution

Michael D. Furtaw, Jon P. Anderson, Lyle R. Middendorf, Gregory R. Bashford

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalPlasmonics
Volume9
Issue number1
DOIs
StatePublished - Feb 2014

Keywords

  • Aggregation
  • Near-infrared fluorescence
  • Plasmonics
  • Silver nanoparticles
  • Surface-enhanced fluorescence

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry

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