TY - JOUR
T1 - Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution
AU - Furtaw, Michael D.
AU - Anderson, Jon P.
AU - Middendorf, Lyle R.
AU - Bashford, Gregory R.
N1 - Funding Information:
Acknowledgments This work was supported by a NSF EPSCoR University-Industry R&D Partnership Award. We would also like to thank John Williams for his review and suggestions.
PY - 2014/2
Y1 - 2014/2
N2 - Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.
AB - Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.
KW - Aggregation
KW - Near-infrared fluorescence
KW - Plasmonics
KW - Silver nanoparticles
KW - Surface-enhanced fluorescence
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U2 - 10.1007/s11468-013-9594-y
DO - 10.1007/s11468-013-9594-y
M3 - Article
AN - SCOPUS:84895064876
SN - 1557-1955
VL - 9
SP - 27
EP - 34
JO - Plasmonics
JF - Plasmonics
IS - 1
ER -