Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution

Michael D. Furtaw; Jon P. Anderson; Lyle R. Middendorf; Gregory R. Bashford

doi:10.1007/s11468-013-9594-y

Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution

Michael D. Furtaw, Jon P. Anderson, Lyle R. Middendorf, Gregory R. Bashford

Biological Systems Engineering

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.

Original language	English (US)
Pages (from-to)	27-34
Number of pages	8
Journal	Plasmonics
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/s11468-013-9594-y
State	Published - Feb 2014

Keywords

Aggregation
Near-infrared fluorescence
Plasmonics
Silver nanoparticles
Surface-enhanced fluorescence

ASJC Scopus subject areas

Biotechnology
Biophysics
Biochemistry

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10.1007/s11468-013-9594-y

Cite this

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title = "Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution",

abstract = "Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.",

keywords = "Aggregation, Near-infrared fluorescence, Plasmonics, Silver nanoparticles, Surface-enhanced fluorescence",

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note = "Funding Information: Acknowledgments This work was supported by a NSF EPSCoR University-Industry R&D Partnership Award. We would also like to thank John Williams for his review and suggestions.",

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AU - Furtaw, Michael D.

AU - Anderson, Jon P.

AU - Middendorf, Lyle R.

AU - Bashford, Gregory R.

N1 - Funding Information: Acknowledgments This work was supported by a NSF EPSCoR University-Industry R&D Partnership Award. We would also like to thank John Williams for his review and suggestions.

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Y1 - 2014/2

N2 - Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.

AB - Fluorescence spectroscopy is used in many life science and clinical research diagnostic assays. Improvements in the sensitivity and limit-of-detection of these assays may have profound implications. Here, we demonstrate a near-infrared, surface-enhanced fluorescence technology that increases the signal of IRDye 800CW-labeled streptavidin by up to 2,530-fold while improving the limit-of-detection 1,000-fold. Citrate-stabilized, silver nanoparticles that aggregate in solution were used with the dye-protein conjugate to form plasmon-active nanostructures. The technique is straightforward to implement and fully compatible with commercially available immunoassay instrumentation and consumables.

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KW - Plasmonics

KW - Silver nanoparticles

KW - Surface-enhanced fluorescence

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Near-Infrared, Surface-Enhanced Fluorescence Using Silver Nanoparticle Aggregates in Solution

Abstract

Keywords

ASJC Scopus subject areas

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