Necrotizing enterocolitis in a neonatal piglet model

Ian T. Cohen, Scarlett D. Nelson, Rodney A. Moxley, Michael P. Hirsh, Timothy C. Counihan, Ronald F. Martin

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The aim of this study was to develop an animal model for necrotizing enterocolitis (NEC). Twenty-five neonatal Hanford minipigs had carotid artery and external jugular vein catheters and rectal Clinical Tonomitors placed under anesthesia. Experimental animals were subjected to a hypoxic insult (50% reduction in baseline PaO2 for 30 minutes) and hypothermic stress (core temperature reduced to 35°C for 30 minutes). Regular oral diet was resumed and the survivors were euthanized 3 to 4 days later. All animals underwent necropsy with gross and histopathological evaluation of the entire bowel. Of 22 experimental animals, 14 survived (64%) and 8 (36%) died of pulmonary hemorrhage. Of the 14 survivors, 8 (57%) had gross and microscopic evidence of NEC. Six of the total 25 animals (24%) sustained rectal perforations from the tonometer. Of 3 control animals, one died of pulmonary hemorrhage and the two survivors had normal intestine. This model successfully produced gross and histological evidence of NEC. The tonometer shows promise as a predictor of NEC provided technical modifications can reduce the complication rate.

Original languageEnglish (US)
Pages (from-to)598-601
Number of pages4
JournalJournal of pediatric surgery
Volume26
Issue number5
DOIs
StatePublished - May 1991

Keywords

  • Necrotizing enterocolitis
  • piglet model

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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    Cohen, I. T., Nelson, S. D., Moxley, R. A., Hirsh, M. P., Counihan, T. C., & Martin, R. F. (1991). Necrotizing enterocolitis in a neonatal piglet model. Journal of pediatric surgery, 26(5), 598-601. https://doi.org/10.1016/0022-3468(91)90716-7