Nematode Molecular Diagnostics: From bands to barcodes

Research output: Contribution to journalReview article

126 Scopus citations

Abstract

Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences of DNA as a "barcode" for the identification of all nematode species.

Original languageEnglish (US)
Pages (from-to)367-383
Number of pages17
JournalAnnual Review of Phytopathology
Volume42
DOIs
StatePublished - Oct 14 2004

Keywords

  • 18S ribosomal DNA
  • DNA barcode
  • Identification
  • Internally transcribed spacer
  • Plant parasitic nematodes

ASJC Scopus subject areas

  • Plant Science

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