TY - JOUR
T1 - Nematode Molecular Diagnostics
T2 - From bands to barcodes
AU - Powers, Tom
PY - 2004
Y1 - 2004
N2 - Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences of DNA as a "barcode" for the identification of all nematode species.
AB - Nematodes are considered among the most difficult animals to identify. DNA-based diagnostic methods have already gained acceptance in applications ranging from quarantine determinations to assessments of biodiversity. Researchers are currently in an information-gathering mode, with intensive efforts applied to accumulating nucleotide sequence of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes. Important linkages with collateral data such as digitized images, video clips and specimen voucher web pages are being established on GenBank and NemATOL, the nematode-specific Tree of Life database. The growing DNA taxonomy of nematodes has lead to their use in testing specific short sequences of DNA as a "barcode" for the identification of all nematode species.
KW - 18S ribosomal DNA
KW - DNA barcode
KW - Identification
KW - Internally transcribed spacer
KW - Plant parasitic nematodes
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U2 - 10.1146/annurev.phyto.42.040803.140348
DO - 10.1146/annurev.phyto.42.040803.140348
M3 - Review article
C2 - 15497206
AN - SCOPUS:4744354028
SN - 0066-4286
VL - 42
SP - 367
EP - 383
JO - Annual Review of Phytopathology
JF - Annual Review of Phytopathology
ER -