Neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells

Angela M. Mann; Makoto Asamoto; Tsuneo Masui; Timothy Macatee; Steven H. Eklund; Samuel M. Cohen

doi:10.1016/0304-3835(94)90352-2

Neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells

Angela M. Mann, Makoto Asamoto, Tsuneo Masui, Timothy Macatee, Steven H. Eklund, Samuel M. Cohen

Research output: Contribution to journal › Article › peer-review

Abstract

Enhanced c-erbB-2/neu expression has been linked with a poor prognosis in human bladder cancer. Previous reports have shown that a point mutation at nucleotide T₂₀₁₂ in the coding region of the transmembrane domain of the rat gene is sufficient to confer transformation potential on this gene. We examined the comparative levels of p185^neu as well as the sequence around the hotspot (T₂₀₁₂) of the neu gene of rat bladder cells transformed by 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) or established in culture from N-[-4-(-5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced rat bladder tumors. We concluded that increased p185^neu expression did not correlate significantly with tumorigenicity. No alterations in nucleotide sequences of the neu gene were observed in either in vitro model.

Original language	English (US)
Pages (from-to)	7-13
Number of pages	7
Journal	Cancer Letters
Volume	84
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(94)90352-2
State	Published - Aug 29 1994

Keywords

Chemical transformation
Neu gene
Rat bladder cells
p185 expression

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells",

abstract = "Enhanced c-erbB-2/neu expression has been linked with a poor prognosis in human bladder cancer. Previous reports have shown that a point mutation at nucleotide T2012 in the coding region of the transmembrane domain of the rat gene is sufficient to confer transformation potential on this gene. We examined the comparative levels of p185neu as well as the sequence around the hotspot (T2012) of the neu gene of rat bladder cells transformed by 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) or established in culture from N-[-4-(-5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced rat bladder tumors. We concluded that increased p185neu expression did not correlate significantly with tumorigenicity. No alterations in nucleotide sequences of the neu gene were observed in either in vitro model.",

keywords = "Chemical transformation, Neu gene, Rat bladder cells, p185 expression",

author = "Mann, {Angela M.} and Makoto Asamoto and Tsuneo Masui and Timothy Macatee and Eklund, {Steven H.} and Cohen, {Samuel M.}",

year = "1994",

month = aug,

day = "29",

doi = "10.1016/0304-3835(94)90352-2",

language = "English (US)",

volume = "84",

pages = "7--13",

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T1 - Neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells

AU - Mann, Angela M.

AU - Asamoto, Makoto

AU - Masui, Tsuneo

AU - Macatee, Timothy

AU - Eklund, Steven H.

AU - Cohen, Samuel M.

PY - 1994/8/29

Y1 - 1994/8/29

N2 - Enhanced c-erbB-2/neu expression has been linked with a poor prognosis in human bladder cancer. Previous reports have shown that a point mutation at nucleotide T2012 in the coding region of the transmembrane domain of the rat gene is sufficient to confer transformation potential on this gene. We examined the comparative levels of p185neu as well as the sequence around the hotspot (T2012) of the neu gene of rat bladder cells transformed by 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) or established in culture from N-[-4-(-5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced rat bladder tumors. We concluded that increased p185neu expression did not correlate significantly with tumorigenicity. No alterations in nucleotide sequences of the neu gene were observed in either in vitro model.

AB - Enhanced c-erbB-2/neu expression has been linked with a poor prognosis in human bladder cancer. Previous reports have shown that a point mutation at nucleotide T2012 in the coding region of the transmembrane domain of the rat gene is sufficient to confer transformation potential on this gene. We examined the comparative levels of p185neu as well as the sequence around the hotspot (T2012) of the neu gene of rat bladder cells transformed by 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT) or established in culture from N-[-4-(-5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-induced rat bladder tumors. We concluded that increased p185neu expression did not correlate significantly with tumorigenicity. No alterations in nucleotide sequences of the neu gene were observed in either in vitro model.

KW - Chemical transformation

KW - Neu gene

KW - Rat bladder cells

KW - p185 expression

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Neu is not involved in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced bladder carcinoma or 2-amino-4-(5-nitro-2-furyl)thiazole transformation of rat bladder epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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