Neuroinflammatory responses from microglia recovered from HIV-1-infected and seronegative subjects

Anuja Ghorpade, Yury Persidsky, Susan Swindells, Kathleen Borgmann, Raisa Persidsky, Spring Holter, Robin Cotter, Howard E. Gendelman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Microglial and macrophage infection and immune activation underlie the pathogenesis of HIV-1-associated dementia (HAD). To assess microglial function in HAD, we isolated cells from brain tissues recovered from an HIV-1-infected patient within 4 h of death. Brain tissue from seronegative patients served as controls. Regional neuropathology was correlated to microglial function. HIV-1-patient microglia formed multinucleated giant cells and produced progeny virions. These microglia secreted reduced basal and LPS-stimulated TNF-α levels compared to controls. Monocytes from seronegative donors paralleled these diminished immune responses following repeated LPS-activation. These results demonstrate changes in innate microglial function following viral infection or chronic immune activation.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalJournal of Neuroimmunology
Volume163
Issue number1-2
DOIs
StatePublished - Jun 2005

Keywords

  • Chronic inflammation
  • HIV-1-associated dementia
  • Microglia activation
  • Rapid autopsy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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