TY - JOUR
T1 - Neuropeptide y enhances ATP-induced formation of inositol phosphates in chromaffin cells
AU - Zheng, Jialin
AU - Zhang, Peijin
AU - Toews, Myron
AU - Hexum, Terry D.
PY - 1997/10/9
Y1 - 1997/10/9
N2 - Bovine chromaffin cells contain high affinity NPY binding sites coupled through a pertussis toxin-sensitive G protein to inhibition of cAMP accumulation. NPY alone does not alter [3H]inositol phosphate formation from [3H]phosphoinositides in these cells. Increasing NPY concentrations, in the presence of ATP (300 μM), produced a dose-dependent enhancement in [3H]inositol phosphate formation, EC50 = 3.2 nM. Inclusion of the selective NPY-Y1 receptor antagonist BW1229 (1 μM) produced a marked decrease in NPY potency (EC50 = 3.3 μM). The Y1 receptor agonist, [Leu31, pro34]NPY, was equally effective with NPY, whereas NPY1836, a Y2 receptor agonist, was much less effective. Inclusion of NPY with ATP also produced an enhancement in the release of intracellular Ca2+. The ability of NPY to enhance both [3H]inositol phosphate formation and the release of intracellular Ca2+ was pertussis toxin-insensitive. NPY action on bovine chromaffin cell receptor(s) appears to facilitated by different G proteins: one which can inhibit cAMP accumulation via a pertussis toxin-sensitive process and another which can enhance ATP activation of the inositol phosphate signaling pathway by a pertussis toxin-insensitive process.
AB - Bovine chromaffin cells contain high affinity NPY binding sites coupled through a pertussis toxin-sensitive G protein to inhibition of cAMP accumulation. NPY alone does not alter [3H]inositol phosphate formation from [3H]phosphoinositides in these cells. Increasing NPY concentrations, in the presence of ATP (300 μM), produced a dose-dependent enhancement in [3H]inositol phosphate formation, EC50 = 3.2 nM. Inclusion of the selective NPY-Y1 receptor antagonist BW1229 (1 μM) produced a marked decrease in NPY potency (EC50 = 3.3 μM). The Y1 receptor agonist, [Leu31, pro34]NPY, was equally effective with NPY, whereas NPY1836, a Y2 receptor agonist, was much less effective. Inclusion of NPY with ATP also produced an enhancement in the release of intracellular Ca2+. The ability of NPY to enhance both [3H]inositol phosphate formation and the release of intracellular Ca2+ was pertussis toxin-insensitive. NPY action on bovine chromaffin cell receptor(s) appears to facilitated by different G proteins: one which can inhibit cAMP accumulation via a pertussis toxin-sensitive process and another which can enhance ATP activation of the inositol phosphate signaling pathway by a pertussis toxin-insensitive process.
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U2 - 10.1006/bbrc.1997.7456
DO - 10.1006/bbrc.1997.7456
M3 - Article
C2 - 9345312
AN - SCOPUS:0031561434
VL - 239
SP - 287
EP - 290
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -