Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

Elisabeth Schmid; Roscoe L. Warner; Larry D. Crouch; Hans P. Friedl; Gerd O. Till; Tony E. Hugli; Peter A. Ward

doi:10.1023/A:1027302017117

Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

Elisabeth Schmid, Roscoe L. Warner, Larry D. Crouch, Hans P. Friedl, Gerd O. Till, Tony E. Hugli, Peter A. Ward

Research output: Contribution to journal › Article › peer-review

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Abstract

Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD 11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.

Original language	English (US)
Pages (from-to)	325-333
Number of pages	9
Journal	Inflammation
Volume	21
Issue number	3
DOIs	https://doi.org/10.1023/A:1027302017117
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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@article{5e0e58e9fefc4786be4259fe7f1325be,

title = "Neutrophil chemotactic activity and C5a following systemic activation of complement in rats",

abstract = "Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD 11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.",

author = "Elisabeth Schmid and Warner, {Roscoe L.} and Crouch, {Larry D.} and Friedl, {Hans P.} and Till, {Gerd O.} and Hugli, {Tony E.} and Ward, {Peter A.}",

note = "Funding Information: Acknowledgments—This work is supported in part by NIH grants GM-29507, GM48477, HL-07517 and AI-31963. The authors would like to thank Beverly Schumann for her assistance in the preparation of the manuscript.",

year = "1997",

doi = "10.1023/A:1027302017117",

language = "English (US)",

volume = "21",

pages = "325--333",

journal = "Inflammation",

issn = "0360-3997",

publisher = "Springer New York",

number = "3",

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TY - JOUR

T1 - Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

AU - Schmid, Elisabeth

AU - Warner, Roscoe L.

AU - Crouch, Larry D.

AU - Friedl, Hans P.

AU - Till, Gerd O.

AU - Hugli, Tony E.

AU - Ward, Peter A.

N1 - Funding Information: Acknowledgments—This work is supported in part by NIH grants GM-29507, GM48477, HL-07517 and AI-31963. The authors would like to thank Beverly Schumann for her assistance in the preparation of the manuscript.

PY - 1997

Y1 - 1997

N2 - Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD 11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.

AB - Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD 11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.

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SN - 0360-3997

VL - 21

SP - 325

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JO - Inflammation

JF - Inflammation

IS - 3

ER -

Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

Abstract

ASJC Scopus subject areas

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