Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

Elisabeth Schmid, Roscoe L. Warner, Larry D. Crouch, Hans P. Friedl, Gerd O. Till, Tony E. Hugli, Peter A. Ward

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD 11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.

Original languageEnglish (US)
Pages (from-to)325-333
Number of pages9
JournalInflammation
Volume21
Issue number3
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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