New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia

Hongxiu Wen, Yongjin Li, Sami N. Malek, Yeong C. Kim, Jia Xu, Peixian Chen, Fengxia Xiao, Xin Huang, Xianzheng Zhou, Zhenyu Xuan, Shiva Mankala, Guihua Hou, Janet D. Rowley, Michael Q. Zhang, San Ming Wang

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Genetic aberrations contribute to acute myeloid leukemia (AML). However, half of AML cases do not contain the well-known aberrations detectable mostly by cytogenetic analysis, and these cases are classified as normal karyotype AML. Different outcomes of normal karyotype AML suggest that this subgroup of AML could be genetically heterogeneous. But lack of genetic markers makes it difficult to further study this subgroup of AML. Using paired-end RNAseq method, we performed a transcriptome analysis in 45 AML cases including 29 normal karyotype AML, 8 abnormal karyotype AML and 8 AML without karyotype informaiton. Our study identified 134 fusion transcripts, all of which were formed between the partner genes adjacent in the same chromosome and distributed at different frequencies in the AML cases. Seven fusions are exclusively present in normal karyotype AML, and the rest fusions are shared between the normal karyotype AML and abnormal karyotype AML. CIITA, a master regulator of MHC class II gene expression and truncated in B-cell lymphoma and Hodgkin disease, is found to fuse with DEXI in 48% of normal karyotype AML cases. The fusion transcripts formed between adjacent genes highlight the possibility that certain such fusions could be involved in oncological process in AML, and provide a new source to identify genetic markers for normal karyotype AML.

Original languageEnglish (US)
Article numbere51203
JournalPloS one
Volume7
Issue number12
DOIs
StatePublished - Dec 12 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Wen, H., Li, Y., Malek, S. N., Kim, Y. C., Xu, J., Chen, P., Xiao, F., Huang, X., Zhou, X., Xuan, Z., Mankala, S., Hou, G., Rowley, J. D., Zhang, M. Q., & Wang, S. M. (2012). New Fusion Transcripts Identified in Normal Karyotype Acute Myeloid Leukemia. PloS one, 7(12), [e51203]. https://doi.org/10.1371/journal.pone.0051203