The γδ TCR is expressed on 1 to 5% of CD3+ human peripheral blood T lymphocytes. The majority of peripheral blood γδ T cells expresses Vγ9 paired with Vδ2; this subset strongly responds to certain microbial ligands. Other γδ T cell subsets with unknown Ag specificity expressing different Vγ elements are present in peripheral blood and lymphoid tissue. We describe a new anti-human Vγ mAb termed 23D12 with unusual specificity. As revealed by analysis of a large number of T cell clones and transfectants expressing molecularly well-defined γδ TCR, mAb 23D12 recognized several, but not all, members of the human Vγ1 family, specifically Vγ2, Vγ3, and Vγ4 but not Vγ5 or Vγ8. In combination with available mAb against Vγ4, mAb 23D12 was used to identify Vγ2- or Vγ3- bearing cells. On average, 23D12+ cells accounted for 18% of peripheral blood γδ T cells and 56% of postnatal γδ thymocytes. In combination with anti-Vγ9 mAb, mAb 23D12 identified γδ cells expressing V elements other than Vγ2, Vγ3, Vγ4, or Vγ9. Such cells are detectable in peripheral blood and postnatal thymus. Using mAb 23D12, we also confirmed the appearance of two distinct TCR γ-chains on the surface of some γδ T cells.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1994|
ASJC Scopus subject areas
- Immunology and Allergy