TY - JOUR
T1 - New restriction endonuclease CviRI cleaves DNA at TG/CA sequences
AU - Jin, Aiwen
AU - Zhang, Yanping
AU - Xia, Yuannan
AU - Traylor, Elbert
AU - Nelson, Michael
AU - van Ettenl, James L.
N1 - Funding Information:
We thank Megabase Research Products Inc. for CViRI restriction endonuclease. This work was supported in part by US Public Health Service grant GM-32441 to JVE and NIGMS grant 1R43-GM48909-01 to Megabase Research Products.
PY - 1994/9/25
Y1 - 1994/9/25
N2 - A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.
AB - A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.
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U2 - 10.1093/nar/22.19.3928
DO - 10.1093/nar/22.19.3928
M3 - Article
C2 - 7937114
AN - SCOPUS:0028041612
SN - 0305-1048
VL - 22
SP - 3928
EP - 3929
JO - Nucleic acids research
JF - Nucleic acids research
IS - 19
ER -