New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

Aiwen Jin, Yanping Zhang, Yuannan Xia, Elbert Traylor, Michael Nelson, James L. van Ettenl

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.

Original languageEnglish (US)
Pages (from-to)3928-3929
Number of pages2
JournalNucleic acids research
Volume22
Issue number19
DOIs
StatePublished - Sep 25 1994

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'New restriction endonuclease CviRI cleaves DNA at TG/CA sequences'. Together they form a unique fingerprint.

Cite this