New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

Aiwen Jin, Yanping Zhang, Yuannan Xia, Elbert Traylor, Michael Nelson, James L. van Ettenl

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.

Original languageEnglish (US)
Pages (from-to)3928-3929
Number of pages2
JournalNucleic acids research
Issue number19
StatePublished - Sep 25 1994

ASJC Scopus subject areas

  • Genetics


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