NF-Y binds to the CCAAT box motif of the FGF-4 gene and promotes FGF-4 expression in embryonal carcinoma cells

Kimberly A. Lamb, Lance R. Johnson, Angie Rizzino

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryo-genesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-4 gene in embryonic cells is regulated by an enhancer in the third exon and by a positive regulatory region upstream of the transcription start site. A CCAAT box motif within the positive regulatory region has been shown to support FGF-4 expression, but the factor that binds to this site in vivo has not been identified. In this report, we demonstrate that the transcription factor complex NF-Y binds to the FGF-4 CCAAT box motif when nuclear extracts from each of the embryonic cell lines and their differentiated cells were examined by gel mobility shift analyses. Importantly, we demonstrate that expression of a dominant-negative NF-YA mutant protein reduces the expression of FGF-4 promoter/reporter gene constructs in F9 EC cells. Hence, we provide strong evidence that the transcription factor NF-Y is involved in the expression of the FGF-4 gene.

Original languageEnglish (US)
Pages (from-to)301-309
Number of pages9
JournalMolecular Reproduction and Development
Volume48
Issue number3
DOIs
StatePublished - Nov 1997

Keywords

  • Cis-regulatory element
  • Embryonal carcinoma
  • Embryonic stem
  • Fibroblast growth factor
  • Gel mobility shift analysis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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