NK1 receptors mediate tachykinin-induced increase in microvascular clearance in hamster cheek pouch

X. P. Gao, R. A. Robbins, R. M. Snider, J. Lowe, S. I. Rennard, P. Anding, I. Rubinstein

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The purpose of this study was to determine the receptor subtype(s) that mediates tachykinin-induced neurogenic plasma extravasation in the hamster cheek pouch. Changes in microvascular clearance were quantified by counting the number of leaky sites and calculating the clearance of fluorescein isothiocyanate-dextran [mol wt 70,000 (Dextran 70)] during suffusion of the cheek pouch with substance P, neurokinin A, neurokinin B, and capsaicin. Suffusion of substance P, capsaicin, and neurokinin A, but not neurokinin B, was associated with a significant concentration-dependent increase in leaky site formation and clearance of fluorescein isothiocyanate-Dextran 70 (P < 0.05). However, the responses to substance P and capsaicin were significantly greater than those to neurokinin A. Pretreatment with the selective, nonpeptide NK1 receptor antagonist, CP-96,345, significantly attenuated substance P- and capsaicin-induced but not neurokinin A-induced responses (P < 0.05). These effects were specific, since the 2R,3R enantiomer, CP-96,344, was inactive, and CP-96,345 had no significant effect on adenosine-induced responses. We conclude that, in the hamster cheek pouch, NK1 receptors are the predominant receptors that mediate neurogenic plasma extravasation.

Original languageEnglish (US)
Pages (from-to)H593-H598
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number2 34-2
StatePublished - 1993


  • CP- 96,345
  • capsaicin
  • dextran
  • microcirculation
  • neurogenic inflammation
  • neuropeptides
  • veins

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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