NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity

Paxton Forgue, Steven Halouska, Mark Werth, Kaimei Xu, Steve Harris, Robert Powers

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We describe a general protocol for using comparative NMR metabolomics data to infer in vivo efficacy, specificity and toxicity of chemical leads within a drug discovery program. The methodology is demonstrated using Aspergillus nidulans to monitor the activity of urate oxidase and orotidine-5′- phosphate decarboxylase and the impact of 8-azaxanthine, an inhibitor of urate oxidase. 8-azaxanthine is shown to inhibit A. nidulans hyphal growth by in vivo inactivation of urate oxidase.

Original languageEnglish (US)
Pages (from-to)1916-1923
Number of pages8
JournalJournal of proteome research
Volume5
Issue number8
DOIs
StatePublished - Aug 2006

Keywords

  • Aspergillus nidulans
  • Drug discovery
  • Metabolomics
  • NMR
  • Principle component analysis
  • Purine degradation pathway
  • Pyrimidine biosynthetic pathway

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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