Non-invasive measurement of effective diffusivities in cell immobilization gels through use of near-infrared spectroscopy

Brenna L. Frazier, Paul Larmour, Mark R. Riley

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Encapsulated cell systems provide some advantages over typical suspension cell cultivations as higher cell densities may be obtained; however, the supply of nutrients to the cells often is a limiting factor in productivity. In this study, we describe the development of a new approach to characterize the effective diffusivity of nutrients in immobilized cell materials. Near-infrared spectroscopy is employed to measure nutrient concentrations within a specially designed diffusion chamber that permits noninvasive sampling at ten spatial positions and multiple timepoints. To demonstrate this technique, we measured the effective diffusivity of glutamine in a cell-free 3% (w/w) agarose gel and determined the effective diffusivity (Deff) = 6.46 × 10-10 m2/s, which is in good agreement with theoretical values.

Original languageEnglish (US)
Pages (from-to)364-368
Number of pages5
JournalBiotechnology and Bioengineering
Volume72
Issue number3
DOIs
StatePublished - Feb 5 2001

Keywords

  • Diffusion
  • Immobilized cells
  • Near-infrared (NIR) spectroscopy
  • Noninvasive measurement

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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