Nonuniform size distribution of nascent peptides: The role of messenger RNA

William G. Chaney; Allan J. Morris

doi:10.1016/0003-9861(78)90414-9

Nonuniform size distribution of nascent peptides: The role of messenger RNA

William G. Chaney, Allan J. Morris

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The origin of the nonuniform size distribution of nascent rabbit globin peptides has been investigated in the reticulocyte lysate and wheat germ cell-free protein synthesis systems. Increasing the concentrations of the cellular components involved in protein synthesis failed to alter the elution pattern observed upon chromatographic analysis of reticulocyte lysate nascent chains. Nascent chains isolated from a globin messenger RNA-directed wheat germ cell-free system showed a nonuniform size distribution of nascent peptides similar to that of the rabbit reticulocyte nascent chains. These observations indicate that the nonuniformity of the globin nascent chains arises from a unique property of the messenger RNA being translated and not from limiting concentrations of a component or components of the reticulocyte protein synthesis system.

Original language	English (US)
Pages (from-to)	734-741
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(78)90414-9
State	Published - Dec 1978
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(78)90414-9

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title = "Nonuniform size distribution of nascent peptides: The role of messenger RNA",

abstract = "The origin of the nonuniform size distribution of nascent rabbit globin peptides has been investigated in the reticulocyte lysate and wheat germ cell-free protein synthesis systems. Increasing the concentrations of the cellular components involved in protein synthesis failed to alter the elution pattern observed upon chromatographic analysis of reticulocyte lysate nascent chains. Nascent chains isolated from a globin messenger RNA-directed wheat germ cell-free system showed a nonuniform size distribution of nascent peptides similar to that of the rabbit reticulocyte nascent chains. These observations indicate that the nonuniformity of the globin nascent chains arises from a unique property of the messenger RNA being translated and not from limiting concentrations of a component or components of the reticulocyte protein synthesis system.",

author = "Chaney, {William G.} and Morris, {Allan J.}",

note = "Funding Information: {\textquoteright} This work was supported by Grant AM-08575 from the United States Public Health Service. A preliminary report of this work has appeared (1).",

year = "1978",

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doi = "10.1016/0003-9861(78)90414-9",

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T1 - Nonuniform size distribution of nascent peptides

T2 - The role of messenger RNA

AU - Chaney, William G.

AU - Morris, Allan J.

N1 - Funding Information: ’ This work was supported by Grant AM-08575 from the United States Public Health Service. A preliminary report of this work has appeared (1).

PY - 1978/12

Y1 - 1978/12

N2 - The origin of the nonuniform size distribution of nascent rabbit globin peptides has been investigated in the reticulocyte lysate and wheat germ cell-free protein synthesis systems. Increasing the concentrations of the cellular components involved in protein synthesis failed to alter the elution pattern observed upon chromatographic analysis of reticulocyte lysate nascent chains. Nascent chains isolated from a globin messenger RNA-directed wheat germ cell-free system showed a nonuniform size distribution of nascent peptides similar to that of the rabbit reticulocyte nascent chains. These observations indicate that the nonuniformity of the globin nascent chains arises from a unique property of the messenger RNA being translated and not from limiting concentrations of a component or components of the reticulocyte protein synthesis system.

AB - The origin of the nonuniform size distribution of nascent rabbit globin peptides has been investigated in the reticulocyte lysate and wheat germ cell-free protein synthesis systems. Increasing the concentrations of the cellular components involved in protein synthesis failed to alter the elution pattern observed upon chromatographic analysis of reticulocyte lysate nascent chains. Nascent chains isolated from a globin messenger RNA-directed wheat germ cell-free system showed a nonuniform size distribution of nascent peptides similar to that of the rabbit reticulocyte nascent chains. These observations indicate that the nonuniformity of the globin nascent chains arises from a unique property of the messenger RNA being translated and not from limiting concentrations of a component or components of the reticulocyte protein synthesis system.

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JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

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ER -

Nonuniform size distribution of nascent peptides: The role of messenger RNA

Abstract

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