TY - JOUR
T1 - Nonviral vectors for in vivo gene delivery
T2 - Physicochemical and pharmacokinetic considerations
AU - Mahato, Ram I.
AU - Takakura, Yoshinobu
AU - Hashida, Mitsuru
PY - 1997
Y1 - 1997
N2 - The use of nonviral vectors is an attractive in vivo gene delivery strategy that is simpler than, and lacks some risks inherent in, viral systems. Liposomes and receptor-mediated polycation systems are promising carriers for delivery and expression of plasmid DNA encoding genes into the target cells. Many barriers need to be overcome for successful in vivo DNA delivery using these carrier systems. Such factors as extent of DNA condensation, particle size of the DNA complex, route of administration, stability against nucleases, target sites, in vivo disposition, binding to cell surface receptor and internalization, and intracellular trafficking affect in vivo gene delivery and expression. This review will provide a critical discussion of the merits and limitations of liposomal and polycationic carrier systems for gene transfer from the viewpoints of their physicochemical and pharmacokinetic properties.
AB - The use of nonviral vectors is an attractive in vivo gene delivery strategy that is simpler than, and lacks some risks inherent in, viral systems. Liposomes and receptor-mediated polycation systems are promising carriers for delivery and expression of plasmid DNA encoding genes into the target cells. Many barriers need to be overcome for successful in vivo DNA delivery using these carrier systems. Such factors as extent of DNA condensation, particle size of the DNA complex, route of administration, stability against nucleases, target sites, in vivo disposition, binding to cell surface receptor and internalization, and intracellular trafficking affect in vivo gene delivery and expression. This review will provide a critical discussion of the merits and limitations of liposomal and polycationic carrier systems for gene transfer from the viewpoints of their physicochemical and pharmacokinetic properties.
KW - gene delivery
KW - liposomes
KW - pharmacokinetics
KW - plasmid DNA
KW - polycation/ligand conjugate
UR - http://www.scopus.com/inward/record.url?scp=0030890713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030890713&partnerID=8YFLogxK
U2 - 10.1615/critrevtherdrugcarriersyst.v14.i2.20
DO - 10.1615/critrevtherdrugcarriersyst.v14.i2.20
M3 - Review article
C2 - 9107520
AN - SCOPUS:0030890713
SN - 0743-4863
VL - 14
SP - 133
EP - 172
JO - Critical Reviews in Therapeutic Drug Carrier Systems
JF - Critical Reviews in Therapeutic Drug Carrier Systems
IS - 2
ER -