Novel Hydrogels of Hyaluronic Acid: Synthesis, Surface Morphology, and Solid-State NMR

Tara Pouyani, Gerard S. Harbison, Glenn D. Prestwich

Research output: Contribution to journalArticlepeer-review

95 Scopus citations


Hyaluronic acid (HA) is a linear polysaccharide consisting of repeating disaccharide units of N-acetyl-D-glucosamine (GlcN Ac) and D-glucuronic acid (GlcUA). A convenient methodology was recently developed that allowed the attachment of pendant hydrazido groups to the glucuronate moieties of hyaluronate oligosaccharides. This methodology was extended to high molecular weight hyaluronate (1.5 × 106), and the products were cross-linked with four homobifunctional activated esters to give novel HA hydrogels. Solid-state 13C NMR using cross-polarization and magic angle spinning (CP-MAS) revealed that the lyophilized native HA and hydrazido-HA retained solution-like structures in the solid state. The four HA hydrogels showed significant structural changes relative to native HA, and the carbon resonances of the cross-linkers were clearly evident. The surface morphologies of these cross-linked HA derivatives were examined using scanning electron microscopy (SEM). The electron micrographs of the freeze-dried hydrogels showed the presence of regular sheetlike structures forming pores (20-50 μm). In contrast, native HA showed predominantly fibrous and irregular structures.

Original languageEnglish (US)
Pages (from-to)7515-7522
Number of pages8
JournalJournal of the American Chemical Society
Issue number17
StatePublished - Aug 1 1994

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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