Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools

Hiromi Miura; Jurai Imafuku; Aki Kurosaki; Masahiro Sato; Yongjie Ma; Guisheng Zhang; Akiko Mizutani; Kenya Kamimura; Channabasavaiah B. Gurumurthy; Dexi Liu; Masato Ohtsuka

doi:10.1016/j.omtn.2021.03.003

Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools

Hiromi Miura, Jurai Imafuku, Aki Kurosaki, Masahiro Sato, Yongjie Ma, Guisheng Zhang, Akiko Mizutani, Kenya Kamimura, Channabasavaiah B. Gurumurthy, Dexi Liu, Masato Ohtsuka

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

CRISPR-Cas is a revolutionary genome-editing technology that is making big impacts in biomedical research and gene therapy. Miura and colleagues developed reporter mice that can emit green fluorescence only in the edited cells or tissues and showed their utility as the much-needed in vivo tools for evaluating CRISPR-Cas technology.

Original language	English (US)
Pages (from-to)	325-336
Number of pages	12
Journal	Molecular Therapy Nucleic Acids
Volume	24
DOIs	https://doi.org/10.1016/j.omtn.2021.03.003
State	Published - Jun 4 2021

Keywords

CRISPR
EGFP
gene therapy
genome editing efficiency
hydrodynamic gene delivery
in vivo gene editing
transgenic mice

ASJC Scopus subject areas

Molecular Medicine
Drug Discovery

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10.1016/j.omtn.2021.03.003

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Miura, H., Imafuku, J., Kurosaki, A., Sato, M., Ma, Y., Zhang, G., Mizutani, A., Kamimura, K., Gurumurthy, C. B., Liu, D., & Ohtsuka, M. (2021). Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools. Molecular Therapy Nucleic Acids, 24, 325-336. https://doi.org/10.1016/j.omtn.2021.03.003

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abstract = "CRISPR-Cas is a revolutionary genome-editing technology that is making big impacts in biomedical research and gene therapy. Miura and colleagues developed reporter mice that can emit green fluorescence only in the edited cells or tissues and showed their utility as the much-needed in vivo tools for evaluating CRISPR-Cas technology.",

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AU - Miura, Hiromi

AU - Imafuku, Jurai

AU - Kurosaki, Aki

AU - Sato, Masahiro

AU - Ma, Yongjie

AU - Zhang, Guisheng

AU - Mizutani, Akiko

AU - Kamimura, Kenya

AU - Gurumurthy, Channabasavaiah B.

AU - Liu, Dexi

AU - Ohtsuka, Masato

PY - 2021/6/4

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AB - CRISPR-Cas is a revolutionary genome-editing technology that is making big impacts in biomedical research and gene therapy. Miura and colleagues developed reporter mice that can emit green fluorescence only in the edited cells or tissues and showed their utility as the much-needed in vivo tools for evaluating CRISPR-Cas technology.

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KW - EGFP

KW - gene therapy

KW - genome editing efficiency

KW - hydrodynamic gene delivery

KW - in vivo gene editing

KW - transgenic mice

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Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools

Abstract

Keywords

ASJC Scopus subject areas

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