@article{f55ee9d01b724d409d9b108e36f6d02c,
title = "Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools",
abstract = "CRISPR-Cas is a revolutionary genome-editing technology that is making big impacts in biomedical research and gene therapy. Miura and colleagues developed reporter mice that can emit green fluorescence only in the edited cells or tissues and showed their utility as the much-needed in vivo tools for evaluating CRISPR-Cas technology.",
keywords = "CRISPR, EGFP, gene therapy, genome editing efficiency, hydrodynamic gene delivery, in vivo gene editing, transgenic mice",
author = "Hiromi Miura and Jurai Imafuku and Aki Kurosaki and Masahiro Sato and Yongjie Ma and Guisheng Zhang and Akiko Mizutani and Kenya Kamimura and Gurumurthy, {Channabasavaiah B.} and Dexi Liu and Masato Ohtsuka",
note = "Funding Information: We thank the staff members in the Support Center for Medical Research and Education, Tokai University (Y. Okada and Y. Iida for their technical support for FACS analysis; Y. Kameyama for tissue section; C. Okada for image analysis; H. Kawata, K. Kitatani, and M. Tanaka for supporting next-generation sequencing; and A. Nakamura for her technical support for zygote microinjection and the oviduct delivery experiment using AsCas12a). We are grateful to Tonya Cejka for English corrections. Alt-R AsCas12a (AsCpf1) Ultra was a gift from IDT. This work was supported by the following grants: The Uehara Memorial Foundation, Japan ; Grant-in-Aid for Scientists (C) ( 18K06040 ) from the Japan Society for the Promotion of Science (JSPS), Japan; Tokai University School of Medicine Research Aid (2015−2016 and 2020) to H.M.; Tokai University School of Medicine Research Aid (2020) to J.I.; and Fund for the Promotion of Joint International Research (Fostering Joint International Research) ( 16KK0189 ) from JSPS, Japan; Nakatani foundation (2019), Japan to M.O. C.B.G. acknowledges the funding support from the NIH (awards R35HG010719 and R21GM129559 ), United States. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = jun,
day = "4",
doi = "10.1016/j.omtn.2021.03.003",
language = "English (US)",
volume = "24",
pages = "325--336",
journal = "Molecular Therapy - Nucleic Acids",
issn = "2162-2531",
publisher = "Nature Publishing Group",
}