TY - JOUR
T1 - Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95
AU - Trujillo, Kelly M.
AU - Yuan, Shyng Shiou F.
AU - Lee, Eva Y.H.P.
AU - Sung, Patrick
PY - 1998/8/21
Y1 - 1998/8/21
N2 - Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes in homologous recombination, telomere length maintenance, and DNA repair processes. Here, we purify from nuclear extract of Raji cells a complex consisting of human Rad50, Mre11, and another protein factor with a size of about 95 kDa (p95), which is likely to be Nibrin, the protein encoded by the gene mutated in Nijmegen breakage syndrome. We show that the Rad50- Mre11-p95 complex possesses manganese-dependent single-stranded DNA endonuclease and 3' to 5' exonuclease activities. These nuclease activities are likely to be important for recombination, repair, and genomic stability.
AB - Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes in homologous recombination, telomere length maintenance, and DNA repair processes. Here, we purify from nuclear extract of Raji cells a complex consisting of human Rad50, Mre11, and another protein factor with a size of about 95 kDa (p95), which is likely to be Nibrin, the protein encoded by the gene mutated in Nijmegen breakage syndrome. We show that the Rad50- Mre11-p95 complex possesses manganese-dependent single-stranded DNA endonuclease and 3' to 5' exonuclease activities. These nuclease activities are likely to be important for recombination, repair, and genomic stability.
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U2 - 10.1074/jbc.273.34.21447
DO - 10.1074/jbc.273.34.21447
M3 - Article
C2 - 9705271
AN - SCOPUS:0032555480
SN - 0021-9258
VL - 273
SP - 21447
EP - 21450
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 34
ER -