TY - JOUR
T1 - Nucleotide excision repair in the third kingdom
AU - Ogrunc, M.
AU - Becker, D. F.
AU - Ragsdale, S. W.
AU - Sancar, A.
PY - 1998
Y1 - 1998
N2 - Nucleotide excision repair, a general repair mechanism for removing DNA damage, is initiated by dual incisions bracketing the lesion. In procaryotes, the dual incisions result in excision of the damage in 12- to 13-nucleotide- long oligomers, and in eucaryotes they result in excision of the damage in the form of 24- to 32-nucleotide-long oligomers. We wished to find out if Archaea perform excision repair. Using cell extracts from Methanobacterium thermoautotrophicum, we found that this organism removes UV-induced (6-4) photoproducts in the form of 10- to 11-mers by incising the sixth to seventh phosphodiester bond 5' to the damage and the fourth phosphodiester bond 3' to the damage.
AB - Nucleotide excision repair, a general repair mechanism for removing DNA damage, is initiated by dual incisions bracketing the lesion. In procaryotes, the dual incisions result in excision of the damage in 12- to 13-nucleotide- long oligomers, and in eucaryotes they result in excision of the damage in the form of 24- to 32-nucleotide-long oligomers. We wished to find out if Archaea perform excision repair. Using cell extracts from Methanobacterium thermoautotrophicum, we found that this organism removes UV-induced (6-4) photoproducts in the form of 10- to 11-mers by incising the sixth to seventh phosphodiester bond 5' to the damage and the fourth phosphodiester bond 3' to the damage.
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U2 - 10.1128/jb.180.21.5796-5798.1998
DO - 10.1128/jb.180.21.5796-5798.1998
M3 - Article
C2 - 9791138
AN - SCOPUS:0031795759
SN - 0021-9193
VL - 180
SP - 5796
EP - 5798
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 21
ER -