Nucleotide excision repair in the third kingdom

M. Ogrunc, D. F. Becker, S. W. Ragsdale, A. Sancar

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Nucleotide excision repair, a general repair mechanism for removing DNA damage, is initiated by dual incisions bracketing the lesion. In procaryotes, the dual incisions result in excision of the damage in 12- to 13-nucleotide- long oligomers, and in eucaryotes they result in excision of the damage in the form of 24- to 32-nucleotide-long oligomers. We wished to find out if Archaea perform excision repair. Using cell extracts from Methanobacterium thermoautotrophicum, we found that this organism removes UV-induced (6-4) photoproducts in the form of 10- to 11-mers by incising the sixth to seventh phosphodiester bond 5' to the damage and the fourth phosphodiester bond 3' to the damage.

Original languageEnglish (US)
Pages (from-to)5796-5798
Number of pages3
JournalJournal of bacteriology
Volume180
Issue number21
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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