Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product

Michael L. Parker, Alan C. Christensen, Albert Boosman, Joseph Stockard, Elton T. Young, A. H. Doermann

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We have determined the nucleotide sequence of gene 23 of bacteriophage T4 by the methods of Maxam and Gilbert and of Sanger. The identities of approximately 80% of the amino acid residues of the major capsid protein which is encoded by gene 23 were determined additionally by Edman degradation of the intact protein and its peptides. Fifteen gene 23 amber mutation sites have been located within the sequence, and the 3′ transcription termination site for genes 21, 22 and 23 has been identified.

Original languageEnglish (US)
Pages (from-to)399-416
Number of pages18
JournalJournal of Molecular Biology
Volume180
Issue number3
DOIs
StatePublished - Dec 15 1984
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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