TY - JOUR
T1 - Nutritional control of dimorphism in Ceratocystis ulmi
AU - Kulkarni, Rajiv K.
AU - Nickerson, Kenneth W.
N1 - Funding Information:
We thank Vance Kramer for his expert technica! assistance. This research was supported by NIH Biomedical Research Support Grant RR 07055. K.W.N. is an NIH Research Career Development Awardee (AI 000327--TMP).
PY - 1981/6
Y1 - 1981/6
N2 - The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are "committed" to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.
AB - The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are "committed" to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.
KW - Ceratocystis ulmi
KW - Dutch elm disease
KW - fungal nitrogen metabolism
KW - yeast-mycelial dimorphism
UR - http://www.scopus.com/inward/record.url?scp=0011155266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0011155266&partnerID=8YFLogxK
U2 - 10.1016/0147-5975(81)90015-3
DO - 10.1016/0147-5975(81)90015-3
M3 - Article
AN - SCOPUS:0011155266
SN - 0147-5975
VL - 5
SP - 148
EP - 154
JO - Experimental Mycology
JF - Experimental Mycology
IS - 2
ER -