Wastewater impoundments at livestock facilties represent a potential source of veterinary pharmaceuticals and steroid hormone contamination to shallow groundwater. This study investigates the occurrence of seventeen veterinary pharmaceuticals and thirteen steroid hormones and hormone metabolites in lagoons and adjacent groundwater at operating swine and beef cattle facilties. Pharmaceutical compounds detected in samples obtained from cattle facilities include sulfamerazine; sulfamethazine; erythromycin; monensin; tiamulin; and sulfathiazole. Lincomycin; ractopamine; sulfamethazine; sulfathiazole; erythromycin; tiamulin and sulfadimethoxine were detected in samples from swine facilities. Steroid hormones were detected less frequently than veterinary pharmaceuticals in this study. Estrone, testosterone, 4-androstenedione, and androsterone were detected in impoundments at concentrations ranging from 0.04 to 3.6 ppb. Estrone and testosterone were infrequently detected in groundwater samples at concentrations up to 0.50 ppb. These data indicate that groundwater underlying livestock facilities is susceptible to contamination by veterinary pharmaceuticals and steroid hormones originating in wastewater lagoons.