Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro

Chen Chen, Daniel Daugherty, Peng Jiang, Wenbin Deng

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Oligodendrocyte progenitor cells (OPCs) in primary culture can give rise to mature oligodendrocytes and type-2, but not type-1 astrocytes depending on the culture conditions. The OPCs thus are called oligodendrocyte-type-2 astrocyte (O2-A) progenitor cells. Mouse embryonic stem cells (mESCs) have been efficiently differentiated into OPCs; however, the fate plasticity of mESC-derived OPCs is not well characterized. In the present study, using GFP-Olig2 mESC line, we showed that the Olig2 +/GFP +/A2B5 +/NG2 + OPCs derived from GFP-Olig2 mESCs can mature into oligodendrocytes when co-cultured with mESC-derived neurons. Interestingly, when induced to astrocytic differentiation by bone morphogenetic protein-4, these mESC-derived OPCs can not only generate type-2 astrocytes, but also type-1 astrocytes. These results challenge the dogma that OPCs in culture can only generate type-2, but not type-1 astrocytes, and support the in vivo finding that during perinatal development, OPCs can give rise to a subset of type-1 astrocytes.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalNeuroscience Letters
Volume523
Issue number2
DOIs
StatePublished - Aug 15 2012

Keywords

  • Mouse embryonic stem cells
  • Neural differentiation
  • Oligodendrocyte progenitor cells
  • Type-1 and type-2 astrocytes

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro'. Together they form a unique fingerprint.

Cite this