TY - JOUR
T1 - Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro
AU - Chen, Chen
AU - Daugherty, Daniel
AU - Jiang, Peng
AU - Deng, Wenbin
N1 - Funding Information:
This work was supported by a postdoctoral fellowship from Shriners Hospitals for Children to Dr. Peng Jiang, and in part supported by grants from National Institutes of Health ( R01NS061983 and R01ES015988 to W.D.), the National Multiple Sclerosis Society (to W.D.), and Shriners Hospitals for Children (to W.D.).
PY - 2012/8/15
Y1 - 2012/8/15
N2 - Oligodendrocyte progenitor cells (OPCs) in primary culture can give rise to mature oligodendrocytes and type-2, but not type-1 astrocytes depending on the culture conditions. The OPCs thus are called oligodendrocyte-type-2 astrocyte (O2-A) progenitor cells. Mouse embryonic stem cells (mESCs) have been efficiently differentiated into OPCs; however, the fate plasticity of mESC-derived OPCs is not well characterized. In the present study, using GFP-Olig2 mESC line, we showed that the Olig2 +/GFP +/A2B5 +/NG2 + OPCs derived from GFP-Olig2 mESCs can mature into oligodendrocytes when co-cultured with mESC-derived neurons. Interestingly, when induced to astrocytic differentiation by bone morphogenetic protein-4, these mESC-derived OPCs can not only generate type-2 astrocytes, but also type-1 astrocytes. These results challenge the dogma that OPCs in culture can only generate type-2, but not type-1 astrocytes, and support the in vivo finding that during perinatal development, OPCs can give rise to a subset of type-1 astrocytes.
AB - Oligodendrocyte progenitor cells (OPCs) in primary culture can give rise to mature oligodendrocytes and type-2, but not type-1 astrocytes depending on the culture conditions. The OPCs thus are called oligodendrocyte-type-2 astrocyte (O2-A) progenitor cells. Mouse embryonic stem cells (mESCs) have been efficiently differentiated into OPCs; however, the fate plasticity of mESC-derived OPCs is not well characterized. In the present study, using GFP-Olig2 mESC line, we showed that the Olig2 +/GFP +/A2B5 +/NG2 + OPCs derived from GFP-Olig2 mESCs can mature into oligodendrocytes when co-cultured with mESC-derived neurons. Interestingly, when induced to astrocytic differentiation by bone morphogenetic protein-4, these mESC-derived OPCs can not only generate type-2 astrocytes, but also type-1 astrocytes. These results challenge the dogma that OPCs in culture can only generate type-2, but not type-1 astrocytes, and support the in vivo finding that during perinatal development, OPCs can give rise to a subset of type-1 astrocytes.
KW - Mouse embryonic stem cells
KW - Neural differentiation
KW - Oligodendrocyte progenitor cells
KW - Type-1 and type-2 astrocytes
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U2 - 10.1016/j.neulet.2012.06.072
DO - 10.1016/j.neulet.2012.06.072
M3 - Article
C2 - 22781495
AN - SCOPUS:84864292159
SN - 0304-3940
VL - 523
SP - 180
EP - 185
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -