On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy

Kenneth P. Roberts; Cheng H. Lin; Maria Singhal; George P. Casale; Gerald J. Small; Ryszard Jankowiak

doi:10.1002/(SICI)1522-2683(20000301)21:4<799::AID-ELPS799>3.0.CO;2-V

On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy

Kenneth P. Roberts, Cheng H. Lin, Maria Singhal, George P. Casale, Gerald J. Small, Ryszard Jankowiak

Research output: Contribution to journal › Article

34 Scopus citations

Abstract

The benzo[a]pyrene (BP)-derived 7-(benzo[a]pyren-6-yl)guanine (BP-6- N7Gua) depurinating one-electron oxidation adduct was identified in the urine extracts of coal-smoke-exposed humans for the first time. Urine samples were prepared by solid-phase extraction and reversed-phase high-performance liquid chromatography. Subsequently, the BP-6-N7Gua adduct was identified on-line with capillary electrophoresis fluorescence line narrowing spectroscopy (CE- FLNS) at 4.2 K. The daily excretion of BP-6-N7Gua in human urine of individuals exposed to coal smoke was approximately 226 pmol per μmol of creatinine. Due to the high level of excretion we propose that BP-6-N7Gua adducts found in urine could serve as effective biomarkers for risk assessment of BP exposure. The results demonstrate that CE-FLNS allows for on-line separation and DNA adducts identification in complex fluid extracts.

Original language	English (US)
Pages (from-to)	799-806
Number of pages	8
Journal	ELECTROPHORESIS
Volume	21
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1522-2683(20000301)21:4<799::AID-ELPS799>3.0.CO;2-V
State	Published - 2000

Keywords

Analyte identification
Capillary electrophoresis
Depurinating DNA adducts
Fluorescence line narrowing
One-electron oxidation

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/(SICI)1522-2683(20000301)21:4<799::AID-ELPS799>3.0.CO;2-V

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Roberts, K. P., Lin, C. H., Singhal, M., Casale, G. P., Small, G. J., & Jankowiak, R. (2000). On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy. ELECTROPHORESIS, 21(4), 799-806. https://doi.org/10.1002/(SICI)1522-2683(20000301)21:4<799::AID-ELPS799>3.0.CO;2-V

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AU - Jankowiak, Ryszard

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