TY - JOUR
T1 - Optical properties and nondestructive estimation of anthocyanin content in plant leaves
AU - Gitelson, Anatoly A.
AU - Merzlyak, Mark N.
AU - Chivkunova, Olga B.
PY - 2001/7/1
Y1 - 2001/7/1
N2 - Absorption and reflectance spectra of maple (Acer platanoides), cotoneaster (Cotoneaster alaunica), dogwood (Cornus alba) and pelargonium (Pelargonium zonale) leaves with a wide range of pigment content and composition were studied in visible and near-infrared spectra in order to reveal specific anthocyanin (Anth) spectral features in leaves. Comparing absorption spectra of Anth-containing and Anth-free leaves with the same chlorophyll (Chl) content, absorption spectra of Anth in leaves were derived. The main spectral feature of Anth absorption in vivo was a peak around 550 nm; the peak magnitude was closely related to Anth content. A quantitative nondestructive technique was developed to subtract Chl contribution to reflectance in this spectral region and retrieve Anth content from reflectance over a wide range of pigment content and composition. Anth reflectance index in the form ARI = (R550)-1 - (R700)-1, where (R550)-1 and (R700)-1 are inverse reflectances at 550 and 700 nm, respectively, allowed an accurate estimation of Anth accumulation, even in minute amounts, in intact senescing and stressed leaves.
AB - Absorption and reflectance spectra of maple (Acer platanoides), cotoneaster (Cotoneaster alaunica), dogwood (Cornus alba) and pelargonium (Pelargonium zonale) leaves with a wide range of pigment content and composition were studied in visible and near-infrared spectra in order to reveal specific anthocyanin (Anth) spectral features in leaves. Comparing absorption spectra of Anth-containing and Anth-free leaves with the same chlorophyll (Chl) content, absorption spectra of Anth in leaves were derived. The main spectral feature of Anth absorption in vivo was a peak around 550 nm; the peak magnitude was closely related to Anth content. A quantitative nondestructive technique was developed to subtract Chl contribution to reflectance in this spectral region and retrieve Anth content from reflectance over a wide range of pigment content and composition. Anth reflectance index in the form ARI = (R550)-1 - (R700)-1, where (R550)-1 and (R700)-1 are inverse reflectances at 550 and 700 nm, respectively, allowed an accurate estimation of Anth accumulation, even in minute amounts, in intact senescing and stressed leaves.
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U2 - 10.1562/0031-8655(2001)074<0038:OPANEO>2.0.CO;2
DO - 10.1562/0031-8655(2001)074<0038:OPANEO>2.0.CO;2
M3 - Article
C2 - 11460535
AN - SCOPUS:0035413205
SN - 0031-8655
VL - 74
SP - 38
EP - 45
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 1
ER -