Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage

Marketa Kaucka; Tomas Zikmund; Marketa Tesarova; Daniel Gyllborg; Andreas Hellander; Josef Jaros; Jozef Kaiser; Julian Petersen; Bara Szarowska; Phillip T. Newton; Vyacheslav Dyachuk; Lei Li; Hong Qian; Anne Sofie Johansson; Yuji Mishina; Joshua D. Currie; Elly M. Tanaka; Alek Erickson; Andrew Dudley; Hjalmar Brismar; Paul Southam; Enrico Coen; Min Chen; Lee S. Weinstein; Ales Hampl; Ernest Arenas; Andrei S. Chagin; Kaj Fried; Igor Adameyko

doi:10.7554/eLife.25902

Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage

Marketa Kaucka, Tomas Zikmund, Marketa Tesarova, Daniel Gyllborg, Andreas Hellander, Josef Jaros, Jozef Kaiser, Julian Petersen, Bara Szarowska, Phillip T. Newton, Vyacheslav Dyachuk, Lei Li, Hong Qian, Anne Sofie Johansson, Yuji Mishina, Joshua D. Currie, Elly M. Tanaka, Alek Erickson, Andrew Dudley, Hjalmar BrismarPaul Southam, Enrico Coen, Min Chen, Lee S. Weinstein, Ales Hampl, Ernest Arenas, Andrei S. Chagin, Kaj Fried, Igor Adameyko

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology. We were able to alter cartilage shape by experimentally manipulating clonal geometries. Using in silico modeling, we discovered that anisotropic proliferation might explain cartilage bending and groove formation at the macro-scale.

Original language	English (US)
Article number	e25902
Journal	eLife
Volume	6
DOIs	https://doi.org/10.7554/eLife.25902
State	Published - Apr 17 2017

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.7554/eLife.25902

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Kaucka, M., Zikmund, T., Tesarova, M., Gyllborg, D., Hellander, A., Jaros, J., Kaiser, J., Petersen, J., Szarowska, B., Newton, P. T., Dyachuk, V., Li, L., Qian, H., Johansson, A. S., Mishina, Y., Currie, J. D., Tanaka, E. M., Erickson, A., Dudley, A., ... Adameyko, I. (2017). Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. eLife, 6, [e25902]. https://doi.org/10.7554/eLife.25902

Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. / Kaucka, Marketa; Zikmund, Tomas; Tesarova, Marketa; Gyllborg, Daniel; Hellander, Andreas; Jaros, Josef; Kaiser, Jozef; Petersen, Julian; Szarowska, Bara; Newton, Phillip T.; Dyachuk, Vyacheslav; Li, Lei; Qian, Hong; Johansson, Anne Sofie; Mishina, Yuji; Currie, Joshua D.; Tanaka, Elly M.; Erickson, Alek; Dudley, Andrew; Brismar, Hjalmar; Southam, Paul; Coen, Enrico; Chen, Min; Weinstein, Lee S.; Hampl, Ales; Arenas, Ernest; Chagin, Andrei S.; Fried, Kaj; Adameyko, Igor.

In: eLife, Vol. 6, e25902, 17.04.2017.

Research output: Contribution to journal › Article › peer-review

Kaucka, M, Zikmund, T, Tesarova, M, Gyllborg, D, Hellander, A, Jaros, J, Kaiser, J, Petersen, J, Szarowska, B, Newton, PT, Dyachuk, V, Li, L, Qian, H, Johansson, AS, Mishina, Y, Currie, JD, Tanaka, EM, Erickson, A, Dudley, A, Brismar, H, Southam, P, Coen, E, Chen, M, Weinstein, LS, Hampl, A, Arenas, E, Chagin, AS, Fried, K & Adameyko, I 2017, 'Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage', eLife, vol. 6, e25902. https://doi.org/10.7554/eLife.25902

Kaucka, Marketa ; Zikmund, Tomas ; Tesarova, Marketa ; Gyllborg, Daniel ; Hellander, Andreas ; Jaros, Josef ; Kaiser, Jozef ; Petersen, Julian ; Szarowska, Bara ; Newton, Phillip T. ; Dyachuk, Vyacheslav ; Li, Lei ; Qian, Hong ; Johansson, Anne Sofie ; Mishina, Yuji ; Currie, Joshua D. ; Tanaka, Elly M. ; Erickson, Alek ; Dudley, Andrew ; Brismar, Hjalmar ; Southam, Paul ; Coen, Enrico ; Chen, Min ; Weinstein, Lee S. ; Hampl, Ales ; Arenas, Ernest ; Chagin, Andrei S. ; Fried, Kaj ; Adameyko, Igor. / Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. In: eLife. 2017 ; Vol. 6.

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abstract = "Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology. We were able to alter cartilage shape by experimentally manipulating clonal geometries. Using in silico modeling, we discovered that anisotropic proliferation might explain cartilage bending and groove formation at the macro-scale.",

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AU - Zikmund, Tomas

AU - Tesarova, Marketa

AU - Gyllborg, Daniel

AU - Hellander, Andreas

AU - Jaros, Josef

AU - Kaiser, Jozef

AU - Petersen, Julian

AU - Szarowska, Bara

AU - Newton, Phillip T.

AU - Dyachuk, Vyacheslav

AU - Li, Lei

AU - Qian, Hong

AU - Johansson, Anne Sofie

AU - Mishina, Yuji

AU - Currie, Joshua D.

AU - Tanaka, Elly M.

AU - Erickson, Alek

AU - Dudley, Andrew

AU - Brismar, Hjalmar

AU - Southam, Paul

AU - Coen, Enrico

AU - Chen, Min

AU - Weinstein, Lee S.

AU - Hampl, Ales

AU - Arenas, Ernest

AU - Chagin, Andrei S.

AU - Fried, Kaj

AU - Adameyko, Igor

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Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage

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