Orotidylate decarboxylase activity in Schizophyllum commune

Concetta C. DiRusso, Charles P. Novotny, Robert C. Ullrich

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Orotidylate decarboxylase (OMP decase) activity was measured in mycelial macerates of Schizophyllum commune by a modification of the method of Beckwith et al., 1962. The uracil-requiring strains used in this study belong to two complementation groups: URA-1 and URA-2. OMP decase activities in ura-2 homokaryons and ura-2 × ura-2 uracil-requiring dikaryons were comparable to levels in wild-type homokaryons and dikaryons. No significant OMP decase activity was detected in ura-1 strains. The ura-1 strains of S. commune may be excellent candidates for transformation with a cloned OMP decase gene from yeast and/or S. commune.

Original languageEnglish (US)
Pages (from-to)90-93
Number of pages4
JournalExperimental Mycology
Volume7
Issue number1
DOIs
StatePublished - Mar 1983

Keywords

  • OMP decarboxylase
  • Schizophyllum commune
  • fungi
  • uracil biosynthesis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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