TY - JOUR
T1 - OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis
AU - Carlson, Kimberly A.
AU - Limoges, Jenae
AU - Pohlman, Garrett D.
AU - Poluektova, Larisa Y.
AU - Langford, Dianne
AU - Masliah, Eliezer
AU - Ikezu, Tsuneya
AU - Gendelman, Howard E.
N1 - Funding Information:
The authors thank Yuri Persidsky, Rod McComb, Phyllis Blease, Anuja Ghorpade, Santhi Gorantla, Spring Holter, Tim Moran, Doug Nieman, Raisa Persidskaia, Polina Poluektova, Shelly Smith, Joel Strominger, Shannon Wakeley and Greg Weber for research and technical support and lively scientific exchanges, and Bruce Trapp and Angela Chang for immunostaining the MS cases. Robin Taylor's support in providing outstanding editorial assistance is most appreciated. The CNND Tissue and Specimen Bank and Susan Morgello of the Manhattan HIV Brain Bank (supported by National Institutes of Health research grant R24MH59724) are thanked for the donation of brain specimens. This work was supported by the Vada Oldfield Research Foundation (T.I.), National Institutes of Health research grants: K08MH01552 (J.L.), R01AI5089401 (T.I.), MH59745, MH45294, DA12065 (E.M.), P01MH57556, P01NS31492, R01NS34239, R37NS36136 (H.E.G.) and NCRR P20RR15635 (T.I. and H.E.G). US patent pending.
PY - 2004/5
Y1 - 2004/5
N2 - OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
AB - OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
KW - Disease markers
KW - HIV-1 encephalitis
KW - Macrophages
KW - Microglia
KW - Transcriptional factor
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U2 - 10.1016/j.jneuroim.2004.01.021
DO - 10.1016/j.jneuroim.2004.01.021
M3 - Article
C2 - 15081264
AN - SCOPUS:1842687445
VL - 150
SP - 186
EP - 198
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -