TY - JOUR
T1 - OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis
AU - Carlson, Kimberly A.
AU - Limoges, Jenae
AU - Pohlman, Garrett D.
AU - Poluektova, Larisa Y.
AU - Langford, Dianne
AU - Masliah, Eliezer
AU - Ikezu, Tsuneya
AU - Gendelman, Howard E.
PY - 2004/5
Y1 - 2004/5
N2 - OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
AB - OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
KW - Disease markers
KW - HIV-1 encephalitis
KW - Macrophages
KW - Microglia
KW - Transcriptional factor
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U2 - 10.1016/j.jneuroim.2004.01.021
DO - 10.1016/j.jneuroim.2004.01.021
M3 - Article
C2 - 15081264
AN - SCOPUS:1842687445
VL - 150
SP - 186
EP - 198
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -