OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis

Kimberly A. Carlson; Jenae Limoges; Garrett D. Pohlman; Larisa Y. Poluektova; Dianne Langford; Eliezer Masliah; Tsuneya Ikezu; Howard E. Gendelman

doi:10.1016/j.jneuroim.2004.01.021

OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis

Kimberly A. Carlson, Jenae Limoges, Garrett D. Pohlman, Larisa Y. Poluektova, Dianne Langford, Eliezer Masliah, Tsuneya Ikezu, Howard E. Gendelman

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.

Original language	English (US)
Pages (from-to)	186-198
Number of pages	13
Journal	Journal of Neuroimmunology
Volume	150
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2004.01.021
State	Published - May 2004

Keywords

Disease markers
HIV-1 encephalitis
Macrophages
Microglia
Transcriptional factor

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis. / Carlson, Kimberly A.; Limoges, Jenae; Pohlman, Garrett D.; Poluektova, Larisa Y.; Langford, Dianne; Masliah, Eliezer; Ikezu, Tsuneya; Gendelman, Howard E.

In: Journal of Neuroimmunology, Vol. 150, No. 1-2, 05.2004, p. 186-198.

Research output: Contribution to journal › Article › peer-review

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abstract = "OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.",

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AU - Pohlman, Garrett D.

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AU - Langford, Dianne

AU - Masliah, Eliezer

AU - Ikezu, Tsuneya

AU - Gendelman, Howard E.

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