OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis

Kimberly A. Carlson, Jenae Limoges, Garrett D. Pohlman, Larisa Y. Poluektova, Dianne Langford, Eliezer Masliah, Tsuneya Ikezu, Howard E. Gendelman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.

Original languageEnglish (US)
Pages (from-to)186-198
Number of pages13
JournalJournal of Neuroimmunology
Volume150
Issue number1-2
DOIs
StatePublished - May 2004

Keywords

  • Disease markers
  • HIV-1 encephalitis
  • Macrophages
  • Microglia
  • Transcriptional factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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