TY - JOUR
T1 - Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging
AU - Shchedrina, Valentina A.
AU - Vorbrüggen, Gerd
AU - Lee, Byung Cheon
AU - Kim, Hwa Young
AU - Kabil, Hadise
AU - Harshman, Lawrence G.
AU - Gladyshev, Vadim N.
N1 - Funding Information:
The authors are very grateful to Dr. Daryl A. Travnicek (Department of Statistics, University of Nebraska-Lincoln, USA) for help with statistical analyses. This work was supported by the NIH (National Institute of Health) grants AG021518 (to VNG) and DK074136 (to LGH), and the KOSEF (Korea Science and Engineering Foundation) grant M10642040001-07N4204-00111 (to HYK).
PY - 2009/7
Y1 - 2009/7
N2 - Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.
AB - Methionine sulfoxide reductases (Msrs) are enzymes that repair oxidized methionine residues in proteins. This function implicated Msrs in antioxidant defense and the regulation of aging. There are two known Msr types in animals: MsrA specific for the reduction of methionine-S-sulfoxide, and MsrB that catalyzes the reduction of methionine-R-sulfoxide. In a previous study, overexpression of MsrA in the nervous system of Drosophila was found to extend lifespan by 70%. Overexpression of MsrA in yeast also extended lifespan, whereas MsrB overexpression did so only under calorie restriction conditions. The effect of MsrB overexpression on lifespan has not yet been characterized in animal model systems. Here, the GAL4-UAS binary system was used to drive overexpression of cytosolic Drosophila MsrB and mitochondrial mouse MsrB2 in whole body, fatbody, and the nervous system of flies. In contrast to MsrA, MsrB overexpression had no consistent effect on the lifespan of fruit flies on either corn meal or sugar yeast diets. Physical activity, fecundity, and stress resistance were also similar in MsrB-overexpressing and control flies. Thus, MsrA and MsrB, the two proteins with similar function in antioxidant protein repair, have different effects on aging in fruit flies.
KW - Aging
KW - Drosophila melanogaster
KW - Lifespan
KW - Methionine sulfoxide reductase
KW - Protein repair
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U2 - 10.1016/j.mad.2009.04.003
DO - 10.1016/j.mad.2009.04.003
M3 - Article
C2 - 19409408
AN - SCOPUS:67349116442
SN - 0047-6374
VL - 130
SP - 429
EP - 443
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 7
ER -