Overexpression of the yeast HAM1 gene prevents 6-N-hydroxylaminopurine mutagenesis in Escherichia coli

Stanislav G. Kozmin, Prune Leroy, Youri I. Pavlov

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have overexpressed yeast Ham1p in E. coli. We demonstrate that under conditions when this protein constitutes approximately 30% of cellular protein, the host strain is protected both from toxic and mutagenic effects of HAP. This result indicates that sole Hamlp activity might be sufficient for destruction of HAP or its metabolites in bacterial cells.

Original languageEnglish (US)
Pages (from-to)645-652
Number of pages8
JournalActa Biochimica Polonica
Volume45
Issue number3
DOIs
StatePublished - 1998

Keywords

  • 6-N-hydroxylaminopurine
  • Base analogue metabolism
  • Escherichia coli
  • Molybdoenzymes
  • Yeast

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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