TY - JOUR
T1 - Oxidative stress in early pregnancy and the risk of preeclampsia
AU - Ahmad, Iman M.
AU - Zimmerman, Matthew C.
AU - Moore, Tiffany A.
N1 - Funding Information:
The authors wish to thank the staff and patients at Nebraska Medicine and Olson Center, Jocelyn Jones, Colton T. Roessner, and Ellen Steffensmeier. This study was funded by NIH 1K01NR014474-01 and a University of Nebraska Medical Center's Edna Ittner Pediatric Support grant. EPR Spectroscopy data was collected in the University of Nebraska's EPR Spectroscopy Core, which was initially established with support from a Center of Biomedical Research Excellence grant from the National Institute of General Medical Sciences of the National Institutes of Health (P30GM103335) awarded to the University of Nebraska's Redox Biology Center.
Funding Information:
This study was funded by NIH 1K01NR014474-01 and a University of Nebraska Medical Center ’s Edna Ittner Pediatric Support grant. EPR Spectroscopy data was collected in the University of Nebraska’s EPR Spectroscopy Core, which was initially established with support from a Center of Biomedical Research Excellence grant from the National Institute of General Medical Sciences of the National Institutes of Health ( P30GM103335 ) awarded to the University of Nebraska’s Redox Biology Center.
Publisher Copyright:
© 2019
PY - 2019/10
Y1 - 2019/10
N2 - Introduction: Preeclampsia (PE), one of the most serious complications of pregnancy, is characterized by endothelial dysfunction and hypertension. The pathophysiology of the disease is still unknown; however, evidence suggests that placental and maternal oxidative stress promote the disease process. Several studies have assessed levels of oxidative stress during pregnancy, but after diagnosis of PE. However, few studies have examined oxidative stress before PE diagnosis. Thus, the present work was aimed to gain further insight into the role of oxidative stress prior to diagnosis of PE (i.e. 12–20 weeks of gestation) and to further understand and predict PE incidence. Methods: Blood levels of superoxide (O2 [rad]−) and erythrocyte antioxidants such as superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were measured in 23 preeclamptic pregnant women and 91 women with normal pregnancies. We further used logistic regression of O2 [rad]− and each antioxidant level as the main predictor variable for PE risk. Results: CAT activity, GSH, and Total glutathione (TGSH) were significantly lower with All PE pregnant groups, whereas O2 [rad]− levels were modestly, but significantly, higher in women with mild PE. Logistic regression analysis suggests increased CAT activity in pregnant women is associated with a decreased odds of being preeclamptic. Conclusion: CAT is the only antioxidant as shown in our study to be related to the severity of the disease and may be a promising predictor for PE. Further studies are warranted to investigate the use of CAT as a novel therapeutic for PE.
AB - Introduction: Preeclampsia (PE), one of the most serious complications of pregnancy, is characterized by endothelial dysfunction and hypertension. The pathophysiology of the disease is still unknown; however, evidence suggests that placental and maternal oxidative stress promote the disease process. Several studies have assessed levels of oxidative stress during pregnancy, but after diagnosis of PE. However, few studies have examined oxidative stress before PE diagnosis. Thus, the present work was aimed to gain further insight into the role of oxidative stress prior to diagnosis of PE (i.e. 12–20 weeks of gestation) and to further understand and predict PE incidence. Methods: Blood levels of superoxide (O2 [rad]−) and erythrocyte antioxidants such as superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were measured in 23 preeclamptic pregnant women and 91 women with normal pregnancies. We further used logistic regression of O2 [rad]− and each antioxidant level as the main predictor variable for PE risk. Results: CAT activity, GSH, and Total glutathione (TGSH) were significantly lower with All PE pregnant groups, whereas O2 [rad]− levels were modestly, but significantly, higher in women with mild PE. Logistic regression analysis suggests increased CAT activity in pregnant women is associated with a decreased odds of being preeclamptic. Conclusion: CAT is the only antioxidant as shown in our study to be related to the severity of the disease and may be a promising predictor for PE. Further studies are warranted to investigate the use of CAT as a novel therapeutic for PE.
KW - Catalase
KW - Early pregnancy
KW - Glutathione
KW - Oxidative stress
KW - Preeclampsia
KW - Superoxide
KW - Superoxide dismutase
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U2 - 10.1016/j.preghy.2019.09.014
DO - 10.1016/j.preghy.2019.09.014
M3 - Article
C2 - 31586785
AN - SCOPUS:85072760070
SN - 2210-7789
VL - 18
SP - 99
EP - 102
JO - Pregnancy Hypertension
JF - Pregnancy Hypertension
ER -