TY - JOUR
T1 - P- and E-cadherin are in separate complexes in cells expressing both cadherins
AU - Johnson, Keith R.
AU - Lewis, Jani E.
AU - Li, Dong
AU - Wahl, James
AU - Soler, Alejandro Peralta
AU - Knudsen, Karen A.
AU - Wheelock, Margaret J.
PY - 1993/8
Y1 - 1993/8
N2 - E- and P-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, are tightly associated with E- and P-cadherin. These proteins appear to link the cadherin to the cytoskeleton and have been proposed to be involved in concentrating cadherins at cell-cell adherens junctions. In this paper we report the production of monoclonal antibodies against both α- and β-catenin and use these antibodies to show that in cells simultaneously expressing two different cadherins, E-cadherin and P-cadherin, each cadherin appears to be present in a separate cadherin/catenin complex.
AB - E- and P-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, are tightly associated with E- and P-cadherin. These proteins appear to link the cadherin to the cytoskeleton and have been proposed to be involved in concentrating cadherins at cell-cell adherens junctions. In this paper we report the production of monoclonal antibodies against both α- and β-catenin and use these antibodies to show that in cells simultaneously expressing two different cadherins, E-cadherin and P-cadherin, each cadherin appears to be present in a separate cadherin/catenin complex.
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U2 - 10.1006/excr.1993.1191
DO - 10.1006/excr.1993.1191
M3 - Article
C2 - 8344378
AN - SCOPUS:0027260419
SN - 0014-4827
VL - 207
SP - 252
EP - 260
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -