P- and E-cadherin are in separate complexes in cells expressing both cadherins

Keith R. Johnson, Jani E. Lewis, Dong Li, James Wahl, Alejandro Peralta Soler, Karen A. Knudsen, Margaret J. Wheelock

Research output: Contribution to journalArticle

119 Scopus citations

Abstract

E- and P-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, are tightly associated with E- and P-cadherin. These proteins appear to link the cadherin to the cytoskeleton and have been proposed to be involved in concentrating cadherins at cell-cell adherens junctions. In this paper we report the production of monoclonal antibodies against both α- and β-catenin and use these antibodies to show that in cells simultaneously expressing two different cadherins, E-cadherin and P-cadherin, each cadherin appears to be present in a separate cadherin/catenin complex.

Original languageEnglish (US)
Pages (from-to)252-260
Number of pages9
JournalExperimental Cell Research
Volume207
Issue number2
DOIs
StatePublished - Aug 1993

ASJC Scopus subject areas

  • Cell Biology

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