PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response

Srilatha Simhadri, Gabriele Vincelli, Yanying Huo, Sarah Misenko, Tzeh Keong Foo, Johanna Ahlskog, Claus S. Sørensen, Gregory G. Oakley, Shridar Ganesan, Samuel F. Bunting, Bing Xia

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The G2/M checkpoint inhibits mitotic entry upon DNA damage, thereby preventing segregation of broken chromosomes and preserving genome stability. The tumor suppressor proteins BRCA1, PALB2 and BRCA2 constitute a BRCA1–PALB2–BRCA2 axis that is essential for homologous recombination (HR)-based DNA doublestrand break repair. Besides HR, BRCA1 has been implicated in both the initial activation and the maintenance of the G2/M checkpoint, while BRCA2 and PALB2 have been shown to be critical for its maintenance. Here we show that all three proteins can play a significant role in both checkpoint activation and checkpoint maintenance, depending on cell type and context, and that PALB2 links BRCA1 and BRCA2 in the checkpoint response. The BRCA1–PALB2 interaction can be important for checkpoint activation, whereas the PALB2–BRCA2 complex formation appears to be more critical for checkpoint maintenance. Interestingly, the function of PALB2 in checkpoint response appears to be independent of CHK1 and CHK2 phosphorylation. Following ionizing radiation, cells with disengaged BRCA1–PALB2 interaction show greatly increased chromosomal abnormalities due apparently to combined defects in HR and checkpoint control. These findings provide new insights into DNA damage checkpoint control and further underscore the critical importance of the proper cooperation of the BRCA and PALB2 proteins in genome maintenance.

Original languageEnglish (US)
Pages (from-to)1585-1596
Number of pages12
JournalOncogene
Volume38
Issue number10
DOIs
StatePublished - Mar 7 2019

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response'. Together they form a unique fingerprint.

  • Cite this

    Simhadri, S., Vincelli, G., Huo, Y., Misenko, S., Foo, T. K., Ahlskog, J., Sørensen, C. S., Oakley, G. G., Ganesan, S., Bunting, S. F., & Xia, B. (2019). PALB2 connects BRCA1 and BRCA2 in the G2/M checkpoint response. Oncogene, 38(10), 1585-1596. https://doi.org/10.1038/s41388-018-0535-2