@article{ff73a2633bf141a58b0712def10addfd,
title = "Palmitoleate protects against Zika virus-induced placental trophoblast apoptosis",
abstract = "Zika virus (ZIKV) infection in pregnancy is associated with the development of microcephaly, intrauterine growth restriction, and ocular damage in the fetus. ZIKV infection of the placenta plays a crucial role in the vertical transmission from the maternal circulation to the fetus. Our previous study suggested that ZIKV induces endoplasmic reticulum (ER) stress and apoptosis of placental trophoblasts. Here, we showed that palmitoleate, an omega-7 monounsaturated fatty acid, prevents ZIKV-induced ER stress and apoptosis in placental trophoblasts. Human trophoblast cell lines (JEG-3 and JAR) and normal immortalized trophoblasts (HTR-8) were used. We observed that ZIKV infection of the trophoblasts resulted in apoptosis and treatment of palmitoleate to ZIKVinfected cells significantly prevented apoptosis. However, palmitate (saturated fatty acid) did not offer protection from ZIKV-induced ER stress and apoptosis. We also observed that the Zika viral RNA copies were decreased, and the cell viability improved in ZIKV-infected cells treated with palmitoleate as compared to the infected cells without palmitoleate treatment. Further, palmitoleate was shown to protect against ZIKV-induced upregulation of ER stress markers, C/EBP homologous protein and X-box binding protein-1 splicing in placental trophoblasts. In conclusion, our studies suggest that palmitoleate protects placental trophoblasts against ZIKV-induced ER stress and apoptosis.",
keywords = "Apoptosis, Endoplasmic reticulum stress, Monounsaturated fatty acids, Placenta, Trophoblast, Viral replication, Zika virus",
author = "Muthuraj, {Philma Glora} and Aryamav Pattnaik and Sahoo, {Prakash K.} and Islam, {Md Torikul} and Pattnaik, {Asit K.} and Byrareddy, {Siddappa N.} and Corrine Hanson and {Anderson Berry}, Ann and Kachman, {Stephen D.} and Natarajan, {Sathish Kumar}",
note = "Funding Information: Funding: The work described was partly supported from Nebraska Center for Prevention of Obesity Disease, National Institute of General Medical Sciences (P20GM104320 to SKN) and by the Nebraska Agricultural Experimental Station, with funding from the Hatch Act (Accession Number 1014526 to SKN) through the United States Department of Agriculture, National Institute of Food and Agriculture (USDA‐NIFA), the Nebraska Tobacco Settlement Biomedical Research Development Funds and the University of Nebraska–Lincoln. The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NIH and NIFA. Funding Information: The work described was partly supported from Nebraska Center for Prevention of Obesity Disease, National Institute of General Medical Sciences (P20GM104320 to SKN) and by the Nebraska Agricultural Experimental Station, with funding from the Hatch Act (Accession Number 1014526 to SKN) through the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA), the Nebraska Tobacco Settlement Biomedical Research Development Funds and the University of Nebraska?Lincoln. The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NIH and NIFA. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = jun,
doi = "10.3390/biomedicines9060643",
language = "English (US)",
volume = "9",
journal = "Biomedicines",
issn = "2227-9059",
publisher = "MDPI AG",
number = "6",
}