TY - JOUR
T1 - Parameters affecting attachment of Bacillus thuringiensis var. israelensis toxin to latex beads
AU - Couche, Graham A.
AU - Pfannenstiel, Mary Ann
AU - Nickerson, Kenneth W.
PY - 1986/5
Y1 - 1986/5
N2 - Bacillus thuringiensis var. israelensis protein toxin radiolabeled with14C-iodoacetamide was bound to latex beads. The efficiency of attachment was not affected by the temperature or length of incubation or by the presence of several different buffers, salts, or organic solvents. However, attachment decreased dramatically at pH values >8.5 or in the presence of detergents (anionic, neutral, or cationic). Protein concentrations ≥2-3 mg/m2 of bead surface led to a progressively decreasing efficiency of protein adsorption. With minor variations, these findings should also be applicable to the attachment of numerous other proteins of diagnostic significance.
AB - Bacillus thuringiensis var. israelensis protein toxin radiolabeled with14C-iodoacetamide was bound to latex beads. The efficiency of attachment was not affected by the temperature or length of incubation or by the presence of several different buffers, salts, or organic solvents. However, attachment decreased dramatically at pH values >8.5 or in the presence of detergents (anionic, neutral, or cationic). Protein concentrations ≥2-3 mg/m2 of bead surface led to a progressively decreasing efficiency of protein adsorption. With minor variations, these findings should also be applicable to the attachment of numerous other proteins of diagnostic significance.
UR - http://www.scopus.com/inward/record.url?scp=85047670238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047670238&partnerID=8YFLogxK
U2 - 10.1007/BF00250060
DO - 10.1007/BF00250060
M3 - Article
AN - SCOPUS:85047670238
SN - 0175-7598
VL - 24
SP - 128
EP - 133
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 2
ER -