Partial characterization of a renotropic factor

D. S. Gaydos; H. Golclin; B. Jenson; D. Gersten; B. Boecleker; C. Bartz; Harry G. Preuss

doi:10.1159/000172892

Partial characterization of a renotropic factor

D. S. Gaydos, H. Golclin, B. Jenson, D. Gersten, B. Boecleker, C. Bartz, Harry G. Preuss

Anesthesiology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of ³H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterallly nephrectomized rats compared to sera from 20-hour sham-operated rats (p<0.001). Dialysis (7.4%, p<0.01) or removal of albumin (11.8%, p<0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p < 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), faileds to enhance DNA synthesis significantly.

Original language	English (US)
Pages (from-to)	139-144
Number of pages	6
Journal	Kidney and Blood Pressure Research
Volume	6
Issue number	3
DOIs	https://doi.org/10.1159/000172892
State	Published - 1983

Keywords

DNA synthesis
Isolated nuclei
Partial characterization
Renal growth
Renotropin

ASJC Scopus subject areas

Nephrology
Cardiology and Cardiovascular Medicine

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10.1159/000172892

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title = "Partial characterization of a renotropic factor",

abstract = "In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of 3H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterallly nephrectomized rats compared to sera from 20-hour sham-operated rats (p<0.001). Dialysis (7.4%, p<0.01) or removal of albumin (11.8%, p<0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p < 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), faileds to enhance DNA synthesis significantly.",

keywords = "DNA synthesis, Isolated nuclei, Partial characterization, Renal growth, Renotropin",

author = "Gaydos, {D. S.} and H. Golclin and B. Jenson and D. Gersten and B. Boecleker and C. Bartz and Preuss, {Harry G.}",

year = "1983",

doi = "10.1159/000172892",

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T1 - Partial characterization of a renotropic factor

AU - Gaydos, D. S.

AU - Golclin, H.

AU - Jenson, B.

AU - Gersten, D.

AU - Boecleker, B.

AU - Bartz, C.

AU - Preuss, Harry G.

PY - 1983

Y1 - 1983

N2 - In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of 3H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterallly nephrectomized rats compared to sera from 20-hour sham-operated rats (p<0.001). Dialysis (7.4%, p<0.01) or removal of albumin (11.8%, p<0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p < 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), faileds to enhance DNA synthesis significantly.

AB - In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of 3H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterallly nephrectomized rats compared to sera from 20-hour sham-operated rats (p<0.001). Dialysis (7.4%, p<0.01) or removal of albumin (11.8%, p<0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p < 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), faileds to enhance DNA synthesis significantly.

KW - DNA synthesis

KW - Isolated nuclei

KW - Partial characterization

KW - Renal growth

KW - Renotropin

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JO - Kidney and Blood Pressure Research

JF - Kidney and Blood Pressure Research

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Partial characterization of a renotropic factor

Abstract

Keywords

ASJC Scopus subject areas

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