Partial derepression of the isoleucine-valine enzymes during methionine starvation in Salmonella typhimurium

Angie Rizzino; Michael Mastanduno; Martin Freundlich

doi:10.1016/0005-2787(77)90017-X

Partial derepression of the isoleucine-valine enzymes during methionine starvation in Salmonella typhimurium

Angie Rizzino, Michael Mastanduno, Martin Freundlich

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Methionine starvation of methionine auxotrophs in the presence of excess branched-chain amino acids results in a partial derepression of the isoleucine and valine enzymes. Reversed-phase chromatography indicated that isoleucine, valine and leucine tRNA were altered during methionine starvation. In addition, the total tRNA isolated from cells under these conditions was undermethylated. The observed derepression may be caused by the inability of methyl-deficient tRNA's to participate adequately in normal regulatory functions.

Original language	English (US)
Pages (from-to)	267-275
Number of pages	9
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	475
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(77)90017-X
State	Published - Mar 18 1977
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Access to Document

10.1016/0005-2787(77)90017-X

Cite this

@article{38ea559f29424bcca4b2f2fc24a7e85d,

title = "Partial derepression of the isoleucine-valine enzymes during methionine starvation in Salmonella typhimurium",

abstract = "Methionine starvation of methionine auxotrophs in the presence of excess branched-chain amino acids results in a partial derepression of the isoleucine and valine enzymes. Reversed-phase chromatography indicated that isoleucine, valine and leucine tRNA were altered during methionine starvation. In addition, the total tRNA isolated from cells under these conditions was undermethylated. The observed derepression may be caused by the inability of methyl-deficient tRNA's to participate adequately in normal regulatory functions.",

author = "Angie Rizzino and Michael Mastanduno and Martin Freundlich",

note = "Funding Information: This work was supported by an NIH research grant (GM 17152) and an NIH Career Development Award (GM 14374) to M.F. The authors are indebted to Ms. K. Chung for excellent technical assistance, Dr. R. Reszelbach for help in the tRNA methylase experiments, and to Dr. M. Iaccarino, in whose laboratory some of these experiments were performed.",

year = "1977",

month = mar,

day = "18",

doi = "10.1016/0005-2787(77)90017-X",

language = "English (US)",

volume = "475",

pages = "267--275",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

issn = "0005-2787",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Partial derepression of the isoleucine-valine enzymes during methionine starvation in Salmonella typhimurium

AU - Rizzino, Angie

AU - Mastanduno, Michael

AU - Freundlich, Martin

N1 - Funding Information: This work was supported by an NIH research grant (GM 17152) and an NIH Career Development Award (GM 14374) to M.F. The authors are indebted to Ms. K. Chung for excellent technical assistance, Dr. R. Reszelbach for help in the tRNA methylase experiments, and to Dr. M. Iaccarino, in whose laboratory some of these experiments were performed.

PY - 1977/3/18

Y1 - 1977/3/18

N2 - Methionine starvation of methionine auxotrophs in the presence of excess branched-chain amino acids results in a partial derepression of the isoleucine and valine enzymes. Reversed-phase chromatography indicated that isoleucine, valine and leucine tRNA were altered during methionine starvation. In addition, the total tRNA isolated from cells under these conditions was undermethylated. The observed derepression may be caused by the inability of methyl-deficient tRNA's to participate adequately in normal regulatory functions.

AB - Methionine starvation of methionine auxotrophs in the presence of excess branched-chain amino acids results in a partial derepression of the isoleucine and valine enzymes. Reversed-phase chromatography indicated that isoleucine, valine and leucine tRNA were altered during methionine starvation. In addition, the total tRNA isolated from cells under these conditions was undermethylated. The observed derepression may be caused by the inability of methyl-deficient tRNA's to participate adequately in normal regulatory functions.

UR - http://www.scopus.com/inward/record.url?scp=0017616952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017616952&partnerID=8YFLogxK

U2 - 10.1016/0005-2787(77)90017-X

DO - 10.1016/0005-2787(77)90017-X

M3 - Article

C2 - 321028

AN - SCOPUS:0017616952

SN - 0005-2787

VL - 475

SP - 267

EP - 275

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 2

ER -

Partial derepression of the isoleucine-valine enzymes during methionine starvation in Salmonella typhimurium

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this