TY - JOUR
T1 - Participation of translesion synthesis DNA polymerases in the maintenance of chromosome integrity in yeast Saccharomyces cerevisiae
AU - Kochenova, O. V.
AU - Soshkina, J. V.
AU - Stepchenkova, E. I.
AU - Inge-Vechtomov, S. G.
AU - Shcherbakova, P. V.
N1 - Funding Information:
This work was supported, in part, by NATO grant CBP.NR.NRCLG 982734, the RFBR grant 09 04 13778 ofi ts, Russian Academy of Sciences Presidium Program “Biodiversity and Dynamic of Genetic Pools”, and by NIH grants ES011644 and ES015869 to P.V.S.
PY - 2011/1
Y1 - 2011/1
N2 - We employed a genetic assay based on illegitimate hybridization of heterothallic Saccharomyces cerevisiae strains (the α-test) to analyze the consequences for genome stability of inactivating translesion synthesis (TLS) DNA polymerases. The α-test is the only assay that measures the frequency of different types of mutational changes (point mutations, recombination, chromosome or chromosome arm loss) and temporary changes in genetic material simultaneously. All these events are manifested as illegitimate hybridization and can be distinguished by genetic analysis of the hybrids and cytoductants. We studied the effect of Polζ, Polη, and Rev1 deficiency on the genome stability in the absence of genotoxic treatment and in UV-irradiated cells. We show that, in spite of the increased percent of accurately repaired primary lesions, chromosome fragility, rearrangements, and loss occur in the absence of Polζ and Polη Our findings contribute to further refinement of the current models of translesion synthesis and the organization of eukaryotic replication fork.
AB - We employed a genetic assay based on illegitimate hybridization of heterothallic Saccharomyces cerevisiae strains (the α-test) to analyze the consequences for genome stability of inactivating translesion synthesis (TLS) DNA polymerases. The α-test is the only assay that measures the frequency of different types of mutational changes (point mutations, recombination, chromosome or chromosome arm loss) and temporary changes in genetic material simultaneously. All these events are manifested as illegitimate hybridization and can be distinguished by genetic analysis of the hybrids and cytoductants. We studied the effect of Polζ, Polη, and Rev1 deficiency on the genome stability in the absence of genotoxic treatment and in UV-irradiated cells. We show that, in spite of the increased percent of accurately repaired primary lesions, chromosome fragility, rearrangements, and loss occur in the absence of Polζ and Polη Our findings contribute to further refinement of the current models of translesion synthesis and the organization of eukaryotic replication fork.
KW - Saccharomyces cerevisiae
KW - chromosome stability
KW - recombination
KW - translesion synthesis
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U2 - 10.1134/S000629791101007X
DO - 10.1134/S000629791101007X
M3 - Article
C2 - 21568839
AN - SCOPUS:79952751825
VL - 76
SP - 49
EP - 60
JO - Biochemistry. Biokhimiia
JF - Biochemistry. Biokhimiia
SN - 0006-2979
IS - 1
ER -