Patterns of aptamers on polyelectrolyte multilayers

Srivatsan Kidambi, Ilsoon Lee, Christina Chan

Research output: Contribution to conferencePaper

Abstract

The ionic layer-by-layer (LBL) assembly technique, introduced by Decher in 1991, is a versatile and inexpensive method of constructing polymeric thin films called "Polyelectrolyte Multilayers (PEMs)", with nanometer-scale control of ionized species. PEMs are excellent candidates for tissue engineering applications due to their biocompatibility and bioinertness, and the ability to incorporate biological molecules, such as proteins. This work describes the successful immobilization and patterning of aptamers on PEM films using electrostatic interaction and covalent crosslinking of nucleic acids on top of thin films. Patterns of thrombin aptamers were formed on PEM surfaces by microcontact printing the nucleic acids onto the PEM surfaces. This technique may be a useful tool for fabricating aptamer arrays to detect and quantitate proteins. Presence and stability of the aptamers on the PEM films were investigated via ellipsometry, FTIR, optical microscope and QCM. Future work will focus on assembling the aptamers to create targeted delivery systems.

Original languageEnglish (US)
Pages4296
Number of pages1
StatePublished - 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Conference

Conference05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Kidambi, S., Lee, I., & Chan, C. (2005). Patterns of aptamers on polyelectrolyte multilayers. 4296. Paper presented at 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States.