Patterns of epoxide metabolism by epoxide hydrolase and glutathione S-transferase associated with age and genotype in Drosophila melanogaster

J. A. Ottea, L. G. Harshman, B. Hammock

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Epoxide hydrolase and glutathione S-transferase activities toward trans- and cis-stilbene oxides were measured in 3 strains of Drosophila melanogaster. Differences in age dependence, substrate selectivity and subcellular location were detected suggesting the presence of multiple forms of these enzymes. In addition, interstrain differences indicate the presence of genetic variation for epoxide hydrolase and glutathione transferase activities. These results illustrate a potential use of these assays in D. melanogaster to complement existing tests (e.g. recessive lethal tests or Ames assays) for evaluating the relationship between epoxide hydrolase and glutathione S-transferase levels and the genotoxicity of epoxides.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume177
Issue number2
DOIs
StatePublished - Apr 1987

Keywords

  • (Drosophila melanogaster)
  • Epoxide hydrolase
  • Genetic variation
  • Glutathione S-transferase
  • Insect epoxide metabolism
  • Isozymes
  • Stilbene oxide

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Patterns of epoxide metabolism by epoxide hydrolase and glutathione S-transferase associated with age and genotype in Drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this