Pedigree assessment using RAPD-DGGE in cereal crop species

Ismail Dweikat, Sally Mackenzie, Morris Levy, Herbert Ohm

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. However, the detection of adequate DNA polymorphism in self-pollinating species remains on obstacle. We have optimized a denaturing-gradient-gel electrophoresis (DGGE) system which, when used in combination with random amplified polymorphic DNA (RAPD) analysis, greatly facilitates the detection of reproducible DNA polymorphism among closely related plant lines. We have used this approach to estimate pedigree relationships among a spectrum of plant materials in wheat, barley and oat. Based on analysis with one or two primers, we were able to distinguish soft from hard winter wheat, and 2-rowed from 6-rowed barley. Further analysis with additional primers allowed resolution of polymorpisms even among closely related lines in highly selected populations. We placed 17 cultivars of oat into two distinct clusters that differed significantly from previous oat pedigree assessments. We believe that DGGE-RAPD is a superior method for detecting DNA polymorphism when compared to RFLP, agarose-RAPD, or polyacrylamide-RAPD methods.

Original languageEnglish (US)
Pages (from-to)497-505
Number of pages9
JournalTheoretical and Applied Genetics
Volume85
Issue number5
DOIs
StatePublished - Jan 1993

Keywords

  • Barley
  • DNA fingerprinting
  • DNA polymorphism
  • Oat
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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